Serial Analysis of Gene Expression (SAGE) is a quantative measurement of gene expression. Data are presented for every cluster contained in the browser window and the selected cluster name is highlighted in red. All data are from the repository at the SageMap project downloaded 26 Jul., 2002. Select the UniGene cluster name to display SageMap's page for that cluster.
SAGE counts are produced by sequencing small "tags" of DNA believed
to be associated with a gene. These tags are produced by attaching poly-A RNA
to oligo-dT beads. After synthesis of double stranded cDNA, transcripts are
cleaved by an anchoring enzyme (usually NlaIII). Then small tags are
produced by ligation with a linker containing a type IIS restriction
enzyme site and cleavage with the tagging enzyme (usually BsmFI). The
tags are then concatenated together and sequenced. The frequency of each
tag is counted and used to infer expression level of transcripts that can
be matched to that tag. All SAGE data presented here were mapped to UniGene
transcripts by the SageMap
project at NCBI.