/projects/compbio/experiments/protein-predict/SAM_T02/scripts/log_user servers@predictioncenter.org /projects/compbio/.html/SAM_T02/results/target02-query-1274285010-30210/T0547.a2m target02-query-1274285010-30210 >> /projects/compbio/.html/SAM_T02/log/logfile /projects/compbio/experiments/protein-predict/SAM_T02/scripts/create_summary_html T0547 http://www.soe.ucsc.edu/projects/compbio/SAM_T02/results/target02-query-1274285010-30210 > summary.html Name "main::BASEURL" used only once: possible typo at /projects/compbio/experiments/protein-predict/SAM_T02/scripts/create_summary_html line 11. chgrp protein summary.html /projects/compbio/experiments/protein-predict/SAM_T02/scripts/add_section_head_summary_html "Inputs and Multiple Alignments" >> summary.html if !(/projects/compbio/experiments/protein-predict/SAM_T02/scripts/clean_a2m T0547.raw > T0547.a2m); \ then echo "

" >> summary.html; \ echo "Error: The submitted sequence was longer than 500 amino acids \ , or else was not an amino acid sequence. We suggest breaking long protein chains into different domains \ with blast or psiblast and submitting the domains separately. Alternatively, you can split long \ proteins into overlapping sections of less than 500 amino acids and submit them separately. Please \ do not submit DNA sequences - our server is not set up to handle them." >> summary.html; \ echo "



" >> summary.html; \ fi Reading /projects/compbio/experiments/models.97/scripts2k/sam-t2k.conf Path: /projects/compbio/experiments/models.97/scripts2k:/projects/compbio/bin/x86_64:/usr/local/bin:/usr/local/gnu/bin:/sbin:/usr/sbin:/bin:/usr/bin SAM: /projects/compbio/experiments/protein-predict/SAM_T02/scripts/../bin_freeze/sam/checkseq v3.5 (July 15, 2005) compiled 05/31/06_11:54:08 Done with sequence reading @@@@ rm -f /projects/compbio/tmp/t02rawinput_9947 Error: Raw file is not in the protein alphabet, or is too long echo Received sequence T0547.a2m Received sequence T0547.a2m /projects/compbio/experiments/protein-predict/SAM_T02/scripts/add_summary_html "Submitted sequence(s)" \ T0547.a2m >> summary.html /projects/compbio/experiments/protein-predict/SAM_T02/bin_freeze/scripts2k/target2k -out T0547.t2k \ -seed T0547.a2m \ -db /scratch/data/nrp/nr \ -tmp_dir /tmp \ -keep_temporary -1 \ -blast_max_report 2000 Reading /projects/compbio/experiments/protein-predict/SAM_T02/bin_freeze/scripts2k/sam-t2k.conf Path: /projects/compbio/experiments/protein-predict/SAM_T02/bin_freeze/scripts2k:/projects/compbio/experiments/protein-predict/SAM_T02/bin_freeze/sam:/usr/local/bin:/usr/local/gnu/bin:/sbin:/usr/sbin:/bin:/usr/bin @@@@ chgrp protein /tmp/target2k-bmecluster-0-6.local-9955 @@@@ cp /projects/compbiotmp/target02-query/target02-query-1274285010-30210/T0547.a2m m0.a2m @@@@ checkseq foo -alphabet protein -db m0.a2m > init.check SAM: checkseq v3.5 (July 15, 2005) compiled 05/31/06_11:54:08 Done with sequence reading @@@@ rm -rf /tmp/target2k-bmecluster-0-6.local-9955 < /dev/null *** Error: /projects/compbio/experiments/protein-predict/SAM_T02/bin_freeze/scripts2k/target2k: m0.a2m has 0 alignment columns A2M format requires upper-case for aligned residues make: *** [T0547.t2k.a2m.gz] Error 9 /projects/compbio/experiments/protein-predict/SAM_T02/scripts/end_section_summary_html >> summary.html /projects/compbio/experiments/protein-predict/SAM_T02/scripts/add_section_head_summary_html \ ' \ Secondary Structure Prediction \ \ (Explanation of secondary-structure predictions) \ ' \ >> summary.html /projects/compbio/experiments/protein-predict/SAM_T02/scripts/end_section_summary_html >> summary.html /projects/compbio/experiments/protein-predict/SAM_T02/scripts/add_section_head_summary_html "Target model scores" >> summary.html /usr/bin/gmake TARGET=T0547 T0547.t2k.100-30-dssp-ebghstl.mlib \ T0547.t2k.100-30-dssp-ebghstl.dist \ T0547.t2k.100-30-dssp-ebghstl-target-scores.rdb \ T0547.t2k.100-30-dssp-ebghstl-target-scores.html \ T0547.t2k.100-30-dssp-ebghstl-target-scores.report gmake[1]: Entering directory `/projects/compbiotmp/target02-query/target02-query-1274285010-30210' /projects/compbio/experiments/protein-predict/SAM_T02/bin_freeze/scripts2k/target2k -out T0547.t2k \ -seed T0547.a2m \ -db /scratch/data/nrp/nr \ -tmp_dir /tmp \ -keep_temporary -1 \ -blast_max_report 2000 Reading /projects/compbio/experiments/protein-predict/SAM_T02/bin_freeze/scripts2k/sam-t2k.conf Path: /projects/compbio/experiments/protein-predict/SAM_T02/bin_freeze/scripts2k:/projects/compbio/experiments/protein-predict/SAM_T02/bin_freeze/sam:/usr/local/bin:/usr/local/gnu/bin:/sbin:/usr/sbin:/bin:/usr/bin @@@@ chgrp protein /tmp/target2k-bmecluster-0-6.local-9979 @@@@ cp /projects/compbiotmp/target02-query/target02-query-1274285010-30210/T0547.a2m m0.a2m @@@@ checkseq foo -alphabet protein -db m0.a2m > init.check SAM: checkseq v3.5 (July 15, 2005) compiled 05/31/06_11:54:08 Done with sequence reading @@@@ rm -rf /tmp/target2k-bmecluster-0-6.local-9979 < /dev/null *** Error: /projects/compbio/experiments/protein-predict/SAM_T02/bin_freeze/scripts2k/target2k: m0.a2m has 0 alignment columns A2M format requires upper-case for aligned residues gmake[1]: *** [T0547.t2k.a2m.gz] Error 9 gmake[1]: Target `T0547.t2k.100-30-dssp-ebghstl.mlib' not remade because of errors. gmake[1]: Target `T0547.t2k.100-30-dssp-ebghstl.dist' not remade because of errors. gmake[1]: Target `T0547.t2k.100-30-dssp-ebghstl-target-scores.rdb' not remade because of errors. gmake[1]: Target `T0547.t2k.100-30-dssp-ebghstl-target-scores.html' not remade because of errors. gmake[1]: Target `T0547.t2k.100-30-dssp-ebghstl-target-scores.report' not remade because of errors. gmake[1]: Leaving directory `/projects/compbiotmp/target02-query/target02-query-1274285010-30210' make: *** [T0547.t2k.100-30-dssp-ebghstl.target-scores] Error 2 /usr/bin/gmake TARGET=T0547 T0547.t2k.100-30-stride-ebghtl.mlib \ T0547.t2k.100-30-stride-ebghtl.dist \ T0547.t2k.100-30-stride-ebghtl-target-scores.rdb \ T0547.t2k.100-30-stride-ebghtl-target-scores.html \ T0547.t2k.100-30-stride-ebghtl-target-scores.report gmake[1]: Entering directory `/projects/compbiotmp/target02-query/target02-query-1274285010-30210' /projects/compbio/experiments/protein-predict/SAM_T02/bin_freeze/scripts2k/target2k -out T0547.t2k \ -seed T0547.a2m \ -db /scratch/data/nrp/nr \ -tmp_dir /tmp \ -keep_temporary -1 \ -blast_max_report 2000 Reading /projects/compbio/experiments/protein-predict/SAM_T02/bin_freeze/scripts2k/sam-t2k.conf Path: /projects/compbio/experiments/protein-predict/SAM_T02/bin_freeze/scripts2k:/projects/compbio/experiments/protein-predict/SAM_T02/bin_freeze/sam:/usr/local/bin:/usr/local/gnu/bin:/sbin:/usr/sbin:/bin:/usr/bin @@@@ chgrp protein /tmp/target2k-bmecluster-0-6.local-9996 @@@@ cp /projects/compbiotmp/target02-query/target02-query-1274285010-30210/T0547.a2m m0.a2m @@@@ checkseq foo -alphabet protein -db m0.a2m > init.check SAM: checkseq v3.5 (July 15, 2005) compiled 05/31/06_11:54:08 Done with sequence reading @@@@ rm -rf /tmp/target2k-bmecluster-0-6.local-9996 < /dev/null *** Error: /projects/compbio/experiments/protein-predict/SAM_T02/bin_freeze/scripts2k/target2k: m0.a2m has 0 alignment columns A2M format requires upper-case for aligned residues gmake[1]: *** [T0547.t2k.a2m.gz] Error 9 gmake[1]: Target `T0547.t2k.100-30-stride-ebghtl.mlib' not remade because of errors. gmake[1]: Target `T0547.t2k.100-30-stride-ebghtl.dist' not remade because of errors. gmake[1]: Target `T0547.t2k.100-30-stride-ebghtl-target-scores.rdb' not remade because of errors. gmake[1]: Target `T0547.t2k.100-30-stride-ebghtl-target-scores.html' not remade because of errors. gmake[1]: Target `T0547.t2k.100-30-stride-ebghtl-target-scores.report' not remade because of errors. gmake[1]: Leaving directory `/projects/compbiotmp/target02-query/target02-query-1274285010-30210' make: *** [T0547.t2k.100-30-stride-ebghtl.target-scores] Error 2 /usr/bin/gmake TARGET=T0547 T0547.t2k.100-30-str.mlib \ T0547.t2k.100-30-str.dist \ T0547.t2k.100-30-str-target-scores.rdb \ T0547.t2k.100-30-str-target-scores.html \ T0547.t2k.100-30-str-target-scores.report gmake[1]: Entering directory `/projects/compbiotmp/target02-query/target02-query-1274285010-30210' /projects/compbio/experiments/protein-predict/SAM_T02/bin_freeze/scripts2k/target2k -out T0547.t2k \ -seed T0547.a2m \ -db /scratch/data/nrp/nr \ -tmp_dir /tmp \ -keep_temporary -1 \ -blast_max_report 2000 Reading /projects/compbio/experiments/protein-predict/SAM_T02/bin_freeze/scripts2k/sam-t2k.conf Path: /projects/compbio/experiments/protein-predict/SAM_T02/bin_freeze/scripts2k:/projects/compbio/experiments/protein-predict/SAM_T02/bin_freeze/sam:/usr/local/bin:/usr/local/gnu/bin:/sbin:/usr/sbin:/bin:/usr/bin @@@@ chgrp protein /tmp/target2k-bmecluster-0-6.local-10012 @@@@ cp /projects/compbiotmp/target02-query/target02-query-1274285010-30210/T0547.a2m m0.a2m @@@@ checkseq foo -alphabet protein -db m0.a2m > init.check SAM: checkseq v3.5 (July 15, 2005) compiled 05/31/06_11:54:08 Done with sequence reading @@@@ rm -rf /tmp/target2k-bmecluster-0-6.local-10012 < /dev/null *** Error: /projects/compbio/experiments/protein-predict/SAM_T02/bin_freeze/scripts2k/target2k: m0.a2m has 0 alignment columns A2M format requires upper-case for aligned residues gmake[1]: *** [T0547.t2k.a2m.gz] Error 9 gmake[1]: Target `T0547.t2k.100-30-str.mlib' not remade because of errors. gmake[1]: Target `T0547.t2k.100-30-str.dist' not remade because of errors. gmake[1]: Target `T0547.t2k.100-30-str-target-scores.rdb' not remade because of errors. gmake[1]: Target `T0547.t2k.100-30-str-target-scores.html' not remade because of errors. gmake[1]: Target `T0547.t2k.100-30-str-target-scores.report' not remade because of errors. gmake[1]: Leaving directory `/projects/compbiotmp/target02-query/target02-query-1274285010-30210' make: *** [T0547.t2k.100-30-str.target-scores] Error 2 /projects/compbio/experiments/protein-predict/SAM_T02/scripts/end_section_summary_html >> summary.html /projects/compbio/experiments/protein-predict/SAM_T02/scripts/add_section_head_summary_html "Template model scores" >> summary.html /projects/compbio/experiments/protein-predict/SAM_T02/bin_freeze/sam/hmmscore T0547.template-lib \ -modellibrary /projects/compbio/experiments/models.97/indexes/t2k-w0.5-db.mlib \ -db_size 17000 \ -db T0547.a2m -rdb 1 \ -select_score 4 -Emax 40 SAM: /projects/compbio/experiments/protein-predict/SAM_T02/bin_freeze/sam/hmmscore v3.5 (July 15, 2005) compiled 06/01/06_09:03:53 Opening T0547.template-lib.dist-rdb for RDB distance output /projects/compbio/experiments/models.97/indexes/t2k-w0.5-db.mlib(2): Reading model 1 (2bcoA.t2k-w0.5.mod) /projects/compbio/experiments/models.97/pdb/2b/2bcoA/nostruct-align/2bcoA.t2k-w0.5.mod(2): Reading nostruct-align/2bcoA.t2k-w0.5-db-tmp model number 1 from MODEL -- Model from alignment file /var/tmp/tmp-build-weighted-model-farm03-3121/tmp.a2m Scoring model 2bcoA.t2k-w0.5.mod (nostruct-align/2bcoA.t2k-w0.5-db-tmp model number 1) Calculating Distances (. = 1000 sequences) % Subsequence-submodel (local) (SW = 2) % Simple scores adjusted by +1.5*ln(seq len) (adjust_score = 2) % Track 0 FIMs added (geometric mean of match probabilities (6)) % Single Track Model: 2bcoA.t2k-w0.5.mod Error: No sequences found in database files. make: *** [T0547.template-lib-scores.rdb] Error 1 /projects/compbio/experiments/protein-predict/SAM_T02/scripts/end_section_summary_html >> summary.html /projects/compbio/experiments/protein-predict/SAM_T02/scripts/add_section_head_summary_html "Top Hits" >> summary.html /projects/compbio/experiments/protein-predict/SAM_T02/scripts/end_section_summary_html >> summary.html /projects/compbio/experiments/protein-predict/SAM_T02/scripts/add_section_head_summary_html "Alignments for Top Hits" >> summary.html /projects/compbio/experiments/protein-predict/SAM_T02/bin_freeze/sam/modelfromalign T0547 \ -alignfile T0547.a2m \ -insert /projects/compbio/lib/fssp-trained.regularizer \ -aweight_bits 0.8 \ -fimtrans -1 \ -fimstrength 1 \ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1 \ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1 \ -aweight_method 1 \ -aweight_exponent 10 Reading parameter file /projects/compbio/lib/fssp-trained.regularizer /projects/compbio/lib/fssp-trained.regularizer(1): Reading REGULARIZER: transitions from FSSP and pseudocounts for diverse weighted HSSP SAM: /projects/compbio/experiments/protein-predict/SAM_T02/bin_freeze/sam/modelfromalign v3.5 (July 15, 2005) compiled 05/31/06_11:54:00 Reading alignment file T0547.a2m (0 sequences, 0 columns) as all positions alignment. No alignment found in alignment file T0547.a2m. Error: No alignment found from which to create a model. make: *** [T0547.mod] Error 1 /projects/compbio/experiments/protein-predict/SAM_T02/scripts/end_section_summary_html >> summary.html /projects/compbio/experiments/protein-predict/SAM_T02/scripts/end_summary_html >> summary.html make: Target `default' not remade because of errors.