CreatePredAlphaCost pred_alpha2k alpha11 T0386.t2k.alpha.rdb 2 alpha alpha_prev sum
CreatePredAlphaCost pred_alpha04 alpha11 T0386.t04.alpha.rdb 2 alpha alpha_prev sum
CreatePredAlphaCost pred_alpha06 alpha11 T0386.t06.alpha.rdb 2 alpha alpha_prev sum

SetCost wet6.5 15 near_backbone 5 way_back 5 dry5 15 dry6.5 20 dry8 15 dry12 5 \
	phobic_fit 2 \
	sidechain 5 \
	n_ca_c	5 bad_peptide 10 \
	bystroff 5 \
	soft_clashes 20  backbone_clashes 2 \
	break 50  \
	pred_alpha2k 2 \
	pred_alpha04 2 \
	pred_alpha06 2 \
	constraints 10 \
	hbond_geom 5 \
	hbond_geom_backbone 10 \
	hbond_geom_beta 50 \
	hbond_geom_beta_pair 100 \
	missing_atoms 1 \
	maybe_metal 0.5 \
	maybe_ssbond 1

// remove maybe_ssbond weight if protein known to be in reducing environment.
// remove maybe_metal weight if protein known to have disulfides or
//	known not to bind metal ions.

// Add the following for extra-cellular proteins:
#	known_ssbond 0.5	# and put ssbond command in constraints file

// For comparative modeling, increase weight of break and hbond_geom...

// put any constraints here, such as helix constraints, strand constraints,
//	sheet constraints, ssbonds, hbonds, or just arbitrary pairs of atoms.

//include T0386.dssp-ehl2.constraints
//include T0386.undertaker-align.sheets
//include rr.constraints

HelixConstraint 	L225	T236	5

Hbond		G237.N	K234.O	2
Hbond		R238.N	M233.O	2
Hbond		L282.N	I279.O	2
Hbond		D294.N	K291.O	2
Hbond		E252.N	K249.O	2
Hbond		Y254.N	V247.O	2
Hbond		V270.N	A273.O	2
Hbond		I275.N	L268.O	2
Hbond		L282.N	I279.O	2
Hbond		D294.N	K291.O	2

# From try1-opt2.sheets
SheetConstraint (T0386)R243 (T0386)K249	(T0386)Y258 (T0386)E252	hbond (T0386)V245	10
SheetConstraint (T0386)G256 (T0386)R259	(T0386)V270 (T0386)A267	hbond (T0386)T257	10
SheetConstraint (T0386)G260 (T0386)G262	(T0386)A267 (T0386)G265	hbond (T0386)G260	10
SheetConstraint (T0386)E264 (T0386)V270	(T0386)I279 (T0386)A273	hbond (T0386)F266	30
SheetConstraint (T0386)L287 (T0386)K291	(T0386)F298 (T0386)D294	hbond (T0386)L287	10
SheetConstraint	A273	G277	K291	L287	hbond	P284	50