CreatePredAlphaCost pred_alpha2k alpha11 T0374.t2k.alpha.rdb 2 alpha alpha_prev sum
CreatePredAlphaCost pred_alpha04 alpha11 T0374.t04.alpha.rdb 2 alpha alpha_prev sum
CreatePredAlphaCost pred_alpha06 alpha11 T0374.t06.alpha.rdb 2 alpha alpha_prev sum

SetCost wet6.5 15 near_backbone 5 way_back 5 dry5 15 dry6.5 20 dry8 15 dry12 5 \
	phobic_fit 2 \
	sidechain 5 \
	n_ca_c	5 bad_peptide 10 \
	bystroff 5 \
	soft_clashes 20  backbone_clashes 2 \
	break 50  \
	pred_alpha2k 2 \
	pred_alpha04 2 \
	pred_alpha06 2 \
	constraints 20 \
	hbond_geom 5 \
	hbond_geom_backbone 10 \
	hbond_geom_beta 50 \
	hbond_geom_beta_pair 100 \
	missing_atoms 1 \
	maybe_metal 0.5 \
	maybe_ssbond 1

// remove maybe_ssbond weight if protein known to be in reducing environment.
// remove maybe_metal weight if protein known to have disulfides or
//	known not to bind metal ions.

// Add the following for extra-cellular proteins:
#	known_ssbond 0.5	# and put ssbond command in constraints file

// For comparative modeling, increase weight of break and hbond_geom...

// put any constraints here, such as helix constraints, strand constraints,
//	sheet constraints, ssbonds, hbonds, or just arbitrary pairs of atoms.

# include T0374.dssp-ehl2.constraints
# include T0374.undertaker-align.sheets
# include rr.constraints

include decoys/try10-opt2.helices


# Altered constraints to (hopefully) remove bulge and form edge strand:
SheetConstraint (T0374)Q2 (T0374)A8	(T0374)D58 (T0374)S52	hbond (T0374)S4	1
SheetConstraint (T0374)R49 (T0374)V56	(T0374)Q69 (T0374)L62	hbond (T0374)G51	1
SheetConstraint (T0374)A55 (T0374)H57	(T0374)L62 (T0374)Q60	hbond (T0374)H57	1
SheetConstraint (T0374)L62 (T0374)Q69	(T0374)V83 (T0374)C76	hbond (T0374)Y68	1
SheetConstraint (T0374)C76 (T0374)N80	(T0374)M113 (T0374)C117	hbond (T0374)L78	1

# New constraint:
SheetConstraint (T0374)Q134 (T0374)R136	(T0374)K114 (T0374)S116	hbond (T0374)R136	1