SetCost wet6.5 15 near_backbone 5 way_back 5 dry5 15 dry6.5 20 dry8 15 dry12 5 \
	phobic_fit 2 \
	sidechain 5 \
	n_ca_c	7 bad_peptide 20 \
	bystroff 5 \
	soft_clashes 40  backbone_clashes 2 \
	break 150  \
	pred_alpha2k 2 \
	pred_alpha04 2 \
	pred_alpha06 2 \
	constraints 10 \
	hbond_geom 5 \
	hbond_geom_backbone 10 \
	hbond_geom_beta 50 \
	hbond_geom_beta_pair 100 \
	missing_atoms 1

KnownBreak	M155

// remove maybe_ssbond weight if protein known to be in reducing environment.
// remove maybe_metal weight if protein known to have disulfides or
//	known not to bind metal ions.

// Add the following for extra-cellular proteins:
#	known_ssbond 0.5	# and put ssbond command in constraints file

// For comparative modeling, increase weight of break and hbond_geom...

// put any constraints here, such as helix constraints, strand constraints,
//	sheet constraints, ssbonds, hbonds, or just arbitrary pairs of atoms.

//include T0370.dssp-ehl2.constraints
//include T0370.undertaker-align.sheets
//include rr.constraints


//from try46-opt2.helices
HelixConstraint (T0370)P4	(T0370)N18	1
HelixConstraint (T0370)S60	(T0370)T69	1
HelixConstraint (T0370)A95	(T0370)T109	1

//from try46-opt2.sheets

SheetConstraint (T0370)V24 (T0370)G32	(T0370)Y45 (T0370)A37	hbond (T0370)A26	2
SheetConstraint (T0370)A26 (T0370)T30	(T0370)I76 (T0370)V72	hbond (T0370)W27	2
SheetConstraint (T0370)L46 (T0370)D48	(T0370)L53 (T0370)T51	hbond (T0370)L46	2
SheetConstraint (T0370)T51 (T0370)A58	(T0370)S126 (T0370)S119	hbond (T0370)F52	2
SheetConstraint (T0370)G70 (T0370)G77	(T0370)A89 (T0370)L82	hbond (T0370)V72	2
SheetConstraint (T0370)R80 (T0370)G87	(T0370)E135 (T0370)R128	hbond (T0370)L82	2
SheetConstraint (T0370)E86 (T0370)L92	(T0370)R128 (T0370)Y122	hbond (T0370)L90	2
SheetConstraint (T0370)R128 (T0370)E135	(T0370)D147 (T0370)S140	hbond (T0370)V130	2
SheetConstraint (T0370)L144 (T0370)R146	(T0370)L151 (T0370)E149	hbond (T0370)R146	2

//from RasMol set pick distance of dimerization interface.  

Constraint	L82.CB	P196.CB	6.1	6.7	2
Constraint	W27.CB	W181.CB	5.9	6.9	2
Constraint	W27.CB	L236.CB	8.8	9.8	2
Constraint	L40.CB	L238.CB	6.2	7.0	2
Constraint	P42.CB	L236.CB	5.9	6.9	2
Constraint	L40.CB	W181.CB	7.2	8.0	2
Constraint	P38.CB	P192.CB	4.7	5.5	2
Constraint	A31.CB	P192.CB	6.1	6.7	2