CreatePredAlphaCost pred_alpha2k alpha11 T0296.t2k.alpha.rdb 2 alpha alpha_prev sum
CreatePredAlphaCost pred_alpha04 alpha11 T0296.t04.alpha.rdb 2 alpha alpha_prev sum
CreatePredAlphaCost pred_alpha06 alpha11 T0296.t06.alpha.rdb 2 alpha alpha_prev sum

SetCost wet6.5 15 near_backbone 5 way_back 5 dry5 15 dry6.5 20 dry8 15 dry12 5 \
	phobic_fit 2 \
	sidechain 5 \
	n_ca_c	5 bad_peptide 10 \
	bystroff 5 \
	soft_clashes 20  backbone_clashes 2 \
	break 50  \
	pred_alpha2k 2 \
	pred_alpha04 2 \
	pred_alpha06 2 \
	constraints 10 \
	hbond_geom 5 \
	hbond_geom_backbone 10 \
	hbond_geom_beta 50 \
	hbond_geom_beta_pair 100 \
	missing_atoms 1 \
	maybe_metal 0.5 \
	maybe_ssbond 1

// remove maybe_ssbond weight if protein known to be in reducing environment.
// remove maybe_metal weight if protein known to have disulfides or
//	known not to bind metal ions.

// Add the following for extra-cellular proteins:
#	known_ssbond 0.5	# and put ssbond command in constraints file

// For comparative modeling, increase weight of break and hbond_geom...

// put any constraints here, such as helix constraints, strand constraints,
//	sheet constraints, ssbonds, hbonds, or just arbitrary pairs of atoms.

# include T0296.dssp-ehl2.constraints
HelixConstraint 	I3	Q16	0.6012
HelixConstraint 	L29	D31	0.6012
HelixConstraint 	I37	E65	0.8423
StrandConstraint	I70	N72	0.7387
StrandConstraint	V75	S76	0.6122
HelixConstraint 	P79	G84	0.6437
HelixConstraint 	T90	E105	0.6566
StrandConstraint	F110	G112	0.6873
HelixConstraint 	D126	A138	0.6774
StrandConstraint	V143	S145	0.6409
HelixConstraint 	M158	T171	0.7696
HelixConstraint 	P216	K225	0.7745
HelixConstraint 	F231	T241	0.7847
HelixConstraint 	Q250	R259	0.6678

StrandConstraint	K181	F185	0.6588
StrandConstraint	V207	N210	0.751
StrandConstraint	G265	D268	0.6816

include rr.0.1.constraints