CASP4 Target T0096

1. Protein Name

FadR

2. Organism Name

E.Coli

3. Number of amino acids (approx)

238

4. Accession number

P09371

5. Sequence Database

Swiss-prot

6. Amino acid sequence

MVIKAQSPAGFAEEYIIESIWNNRFPPGTIL
PAERELSELIGVTRTTLREVLQRLARDGWL
TIQHGKPTKVNNFWETSGLNILETLARLDH
ESVPQLIDNLLSVRTNISTIFIRTAFRQHP
DKAQEVLATANEVADHADAFAELDYNIFRG
LAFASGNPIYGLILNGMKGLYTRIGRHYFA
NPEARSLALGFYHKLSALCSEGAHDQVYET
VRRYGHESGEIWHRMQKNLPGDLAIQGR

7. Additional Information

Two domain protein: DNA binding and acyl-coenzyme A binding.
The latter domain has a novel fold (no significant DALI hits).
Site of acyl-CoA binding known from mutations, yet the
structure determination concerns the apo form, so this
could be an interesting ab-inito structure determination
target for the last domain, but also a docking target to
find the binding site (if you can predict the structure
acurately, that is :-) ).
The protein is a HOMODIMER, *both* in presence and absence
of DNA. Binding of acyl-CoA presumably causes a conformational
change, switching off DNA binding and thus transcriptional
respression.
For 8. below: weak homology to HTH DNA binding domains for
the first 72 residues of the structure. No homology for
C-terminal domain.

8. Homologous Sequence of known structure

no

9. Current state of the experimental work

Structure solved last month by MAD. Refinement done.
Paper submitted.

10. Interpretable map?

yes

11. Estimated date of chain tracing completion

June 1 2000

12. Estimated date of public release of structure

end 2000

13. Name

Unavailable until after public release of structure