make -k T0087-blast.rdb T0087-double-blast.rdb make[1]: Entering directory `/auto/projects/compbio/experiments/casp4/t87' /projects/compbio/experiments/casp3/scripts/single-blast -q T0087.seq -interdb /projects/compbio/data/nrp/nr -db /projects/compbio/data/pdb/all-protein > T0087-blast.rdb starting T0087 with E=10 /projects/compbio/experiments/casp3/scripts/double-blast -q T0087.seq -interdb /projects/compbio/data/nrp/nr -db /projects/compbio/data/pdb/all-protein > T0087-double-blast.rdb starting T0087 with E=0.000050 T0087 T0087 blasting: T0087 gi|586817|sp|P37487|YYBQ_BACSU_1:306 blasting: T0087 gi|2496072|sp|Q58025|Y608_METJA_4:307 blasting: T0087 gi|3024962|sp|Q58395|Y988_METJA_11:163 blasting: T0087 gi|3025197|sp|Q59027|YG33_METJA_167:326 blasting: T0087 gi|1743856|gb|AAB39104.1|_1:310 blasting: T0087 gi|7447473|pir||D69344_5:319 blasting: T0087 gi|7674163|sp|O68579|PPX1_STRMU_1:310 blasting: T0087 gi|7445472|pir||F70440_19:208 blasting: T0087 gi|7514584|pir||D71323_46:233 blasting: T0087 gi|7462213|pir||D72359_301:561 blasting: T0087 gi|7462213|pir||D72359_23:82 blasting: T0087 gi|7471265|pir||A75258_2:310 blasting: make[1]: Leaving directory `/auto/projects/compbio/experiments/casp4/t87' make -k T0087.t2k.a2m.gz \ T0087.t2k.pa \ T0087.t2k.pa.html \ T0087.t2k.2d \ T0087.t2k.tree \ T0087.t2k_sorted.pa \ T0087.t2k.tree-unroot.ps make[1]: Entering directory `/auto/projects/compbio/experiments/casp4/t87' /projects/compbio/experiments/models.97/scripts2k/target2k -out T0087.t2k \ -seed T0087.seq \ -all @@@@ chgrp protein /projects/compbio/tmp/target2k-beta-30251 @@@@ cp /projects/compbio/experiments/casp4/t87/T0087.seq m0.a2m @@@@ checkseq foo -alphabet protein -db m0.a2m > init.check SAM: checkseq v3.1b (February 24, 1999) compiled 04/18/00_11:44:44 # About to count sequences in /projects/compbio/data/nrp/nr make[1]: *** [T0087.t2k.a2m.gz] Terminated make: *** [T0087.align] Terminated make -k T0087-blast.rdb T0087-double-blast.rdb make[1]: Entering directory `/auto/projects/compbio/experiments/casp4/t87' make[1]: `T0087-blast.rdb' is up to date. make[1]: `T0087-double-blast.rdb' is up to date. make[1]: Leaving directory `/auto/projects/compbio/experiments/casp4/t87' make -k T0087.t2k.a2m.gz \ T0087.t2k.pa \ T0087.t2k.pa.html \ T0087.t2k.2d \ T0087.t2k.tree \ T0087.t2k_sorted.pa \ T0087.t2k.tree-unroot.ps make[1]: Entering directory `/auto/projects/compbio/experiments/casp4/t87' /projects/compbio/experiments/models.97/scripts2k/target2k -out T0087.t2k \ -seed T0087.seq \ -all @@@@ chgrp protein /projects/compbio/tmp/target2k-beta-3921 @@@@ cp /projects/compbio/experiments/casp4/t87/T0087.seq m0.a2m @@@@ checkseq foo -alphabet protein -db m0.a2m > init.check SAM: checkseq v3.1b (February 24, 1999) compiled 04/18/00_11:44:44 # About to count sequences in /projects/compbio/data/nrp/nr /projects/compbio/experiments/models.97/scripts2k/target2k: E-value thresholds used are prefilter: 0.01, actual: 0.0001 prefilter: 1, actual: 0.0002 prefilter: 10, actual: 0.001 prefilter: 400, actual: 0.005 /projects/compbio/experiments/models.97/scripts2k/target2k: aweight_bits are 0.8, 0.7, 0.6, 0.5 /projects/compbio/experiments/models.97/scripts2k/target2k: db_size= 499925 reverse_diff= 4 /projects/compbio/experiments/models.97/scripts2k/target2k: blast_max_report= 20000 /projects/compbio/experiments/models.97/scripts2k/target2k: db=/projects/compbio/data/nrp/nr /projects/compbio/experiments/models.97/scripts2k/target2k: aweight_method=1 aweight_exponent=10 /projects/compbio/experiments/models.97/scripts2k/target2k: force_seed=1 constraints=1 /projects/compbio/experiments/models.97/scripts2k/target2k: jump_in=0.2 jump_out=1 fimstrength=1 fimtrans=-1 @@@@ blast-prefilter -out prefilter -seed m0.a2m -prefilter_thresholds 0.01,1,10,400 -db /projects/compbio/data/nrp/nr -blast_max_report 20000 @@@@ chgrp protein /projects/compbio/tmp/blast-prefilter-beta-29872 /projects/compbio/experiments/models.97/scripts2k/blast-prefilter: E-value thresholds used are For /projects/compbio/tmp/target2k-beta-3921/prefilter_1.fa, 0.01 For /projects/compbio/tmp/target2k-beta-3921/prefilter_2.fa, 1 For /projects/compbio/tmp/target2k-beta-3921/prefilter_3.fa, 10 For /projects/compbio/tmp/target2k-beta-3921/prefilter_4.fa, 400 /projects/compbio/experiments/models.97/scripts2k/blast-prefilter: db=/projects/compbio/data/nrp/nr @@@@ uniqueseq init -alignfile /projects/compbio/tmp/target2k-beta-3921/m0.a2m -a protein -a2mdots 0 SAM: uniqueseq v3.1b (February 24, 1999) compiled 04/18/00_11:44:50 Reading alignment file /projects/compbio/tmp/target2k-beta-3921/m0.a2m (1 sequences, 310 columns) as A2M alignment. Writing sequence output to init.a2m. No sequences have been droppd. @@@@ /projects/compbio/bin/wu-blastp /projects/compbio/data/nrp/nr 1.fasta E=400 V=20000 B=0 -gi > 1.fasta-blast.out Searched 499925 sequences # parsing wu-blastp output @@@@ rm -f 1.fasta < /dev/null @@@@ rm -f 1.fasta-blast.out < /dev/null # Extracting hits in FASTA format from /projects/compbio/data/nrp/nr @@@@ rm -rf /projects/compbio/tmp/blast-prefilter-beta-29872 < /dev/null @@@@ modelfromalign unused -alignfile m0.a2m \ -constraints_from_align 1 -constraints_out m0.cst SAM: modelfromalign v3.1b (February 24, 1999) compiled 04/18/00_11:44:13 Reading alignment file m0.a2m (1 sequences, 310 columns) as A2M alignment. # About to count sequences in m0.a2m # About to count sequences in prefilter_1.fa @@@@ modelfromalign tmp_1-a -alignfile m0.a2m \ -insert /projects/compbio/lib/gap1.5.regularizer \ -aweight_bits 0.8\ -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1 \ -aweight_method 1\ -aweight_exponent 10 -constraints m0.cst -constraints_out tmp_1-a.cst Reading parameter file /projects/compbio/lib/gap1.5.regularizer /projects/compbio/lib/gap1.5.regularizer(1): Reading REGULARIZER: gap1.5. Weak regularizer, gaps average 1.5, matches average 10 SAM: modelfromalign v3.1b (February 24, 1999) compiled 04/18/00_11:44:13 Reading alignment file m0.a2m (1 sequences, 310 columns) as A2M alignment. @@@@ hmmscore tmp_1-a -i tmp_1-a.mod -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1\ -dpstyle 0 \ -subtract_null 4 -sort 2 \ -db_size 499925 \ -simple_threshold -26.3325537379294 -Emax 0.0001 \ -adpstyle 1 \ -align_short 0 -mdEmax 0.0001\ -select_score 4 \ -select_seq 4 \ -select_mdalign 4 -select_md 4 -Motifcutoff 0.0645161290322581 -db prefilter_1.fa -constraints tmp_1-a.cst -constraints_out tmp_1-a-md.cst Reading parameter file tmp_1-a.mod tmp_1-a.mod(23): Reading MODEL -- Model from alignment file m0.a2m SAM: hmmscore v3.1b (February 24, 1999) compiled 04/18/00_11:44:03 Calculating Distances (. = 1000 sequences) . Average NLL-Simple NULL score: -135.394501 Database has 16 sequences with 5986 residues. @@@@ sortseq tmp_1-a-sort -alignfile tmp_1-a.mult \ -NLLfile tmp_1-a.mstat -a2mdots 0 SAM: sortseq v3.1b (February 24, 1999) compiled 04/18/00_11:44:38 Reading alignment file tmp_1-a.mult (11 sequences, 310 columns) as A2M alignment. Reading scores from file tmp_1-a.mstat Writing sequence output to tmp_1-a-sort.a2m. @@@@ cat m0.a2m tmp_1-a-sort.a2m > tmp_1-a.train.seq @@@@ cat m0.cst tmp_1-a-md.cst > tmp_1-a.train.cst # About to count sequences in tmp_1-a.train.seq # num_seqs=12 nll_thresh=-22.3325537379294 frac_id=0.999999993382876 @@@@ uniqueseq thinned_tmp -alignfile tmp_1-a.train.seq -a protein \ -percent_id 0.999999993382876 -a2mdots 0 SAM: uniqueseq v3.1b (February 24, 1999) compiled 04/18/00_11:44:50 Reading alignment file tmp_1-a.train.seq (12 sequences, 310 columns) as A2M alignment. Writing sequence output to thinned_tmp.a2m. Dropping 1 (of 12) duplicate sequences with differing IDs 11 sequences left after dropping 1 of 12 sequences. @@@@ modelfromalign junk_for_weights \ -alignfile tmp_1-a.train.seq \ -aweight_bits 0.8\ -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1 \ -aweight_method 1\ -aweight_exponent 10 SAM: modelfromalign v3.1b (February 24, 1999) compiled 04/18/00_11:44:13 Reading alignment file tmp_1-a.train.seq (11 sequences, 310 columns) as A2M alignment. @@@@ buildmodel tmp_1-b -insert tmp_1-a.mod\ -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1\ -modellength 0 \ -internal_weight 0 \ -anneal_length 2 \ -many_files 1 \ -Nseq 20000\ -nsurgery 0 \ -fracinsert 0.2 \ -cutinsert 0.25 -cutmatch 0.2 -initial_noise 0.0001 -anneal_noise 0.000001 \ -internal_weight 0 \ -train tmp_1-a.train.seq \ -nmodels 1\ -insert /projects/compbio/lib/gap1.5.regularizer -sequence_weights tmp_1-a.w -constraints tmp_1-a.train.cst Reading parameter file tmp_1-a.mod tmp_1-a.mod(23): Reading MODEL -- Model from alignment file m0.a2m Command line option(48): internal_weight has already been set. Using 0. Reading parameter file /projects/compbio/lib/gap1.5.regularizer /projects/compbio/lib/gap1.5.regularizer(1): Reading REGULARIZER: gap1.5. Weak regularizer, gaps average 1.5, matches average 10 SAM: buildmodel v3.1b (February 24, 1999) compiled 04/18/00_11:43:59 -867.35 95.65 -273.59 253.27 21 0 311 @@@@ hmmscore tmp_1-b -i tmp_1-b.mod -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1\ -dpstyle 0 \ -subtract_null 4 -sort 2 \ -db_size 499925 \ -db m0.a2m -db tmp_1-a.sel \ -select_score 4 \ -select_seq 4 \ -simple_threshold -26.3325537379294 -Emax 0.0001 \ -select_mdalign 4 -select_md 4 \ -adpstyle 1 \ -Motifcutoff 0.0645161290322581 \ -align_short 5 -mdEmax 0.0001 -constraints tmp_1-a.train.cst -constraints_out /projects/compbio/experiments/casp4/t87/T0087.t2k_1.cst Reading parameter file tmp_1-b.mod tmp_1-b.mod(20): Reading MODEL -- Final model for run tmp_1-b SAM: hmmscore v3.1b (February 24, 1999) compiled 04/18/00_11:44:03 Calculating Distances (. = 1000 sequences) . Average NLL-Simple NULL score: -276.446991 Database has 14 sequences with 5007 residues. @@@@ cp -f tmp_1-b.mult /projects/compbio/experiments/casp4/t87/T0087.t2k_1.a2m # About to count sequences in prefilter_2.fa @@@@ modelfromalign tmp_2-a -alignfile /projects/compbio/experiments/casp4/t87/T0087.t2k_1.a2m \ -insert /projects/compbio/lib/stiff-gap5.regularizer \ -aweight_bits 0.7\ -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1 \ -aweight_method 1\ -aweight_exponent 10 -constraints /projects/compbio/experiments/casp4/t87/T0087.t2k_1.cst -constraints_out tmp_2-a.cst Reading parameter file /projects/compbio/lib/stiff-gap5.regularizer /projects/compbio/lib/stiff-gap5.regularizer(1): Reading REGULARIZER: gap5. stiff regularizer, gaps averge 5, matches average 10 SAM: modelfromalign v3.1b (February 24, 1999) compiled 04/18/00_11:44:13 Reading alignment file /projects/compbio/experiments/casp4/t87/T0087.t2k_1.a2m (15 sequences, 310 columns) as A2M alignment. @@@@ hmmscore tmp_2-a -i tmp_2-a.mod -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1\ -dpstyle 0 \ -subtract_null 4 -sort 2 \ -db_size 499925 \ -simple_threshold -25.6394065571694 -Emax 0.0002 \ -adpstyle 1 \ -align_short 0 -mdEmax 0.0002\ -select_score 4 \ -select_seq 4 \ -select_mdalign 4 -select_md 4 -Motifcutoff 0.0645161290322581 -db prefilter_2.fa -constraints tmp_2-a.cst -constraints_out tmp_2-a-md.cst Reading parameter file tmp_2-a.mod tmp_2-a.mod(23): Reading MODEL -- Model from alignment file /projects/compbio/experiments/casp4/t87/T0087.t2k_1.a2m SAM: hmmscore v3.1b (February 24, 1999) compiled 04/18/00_11:44:03 Calculating Distances (. = 1000 sequences) . Average NLL-Simple NULL score: -137.702866 Database has 31 sequences with 12217 residues. @@@@ sortseq tmp_2-a-sort -alignfile tmp_2-a.mult \ -NLLfile tmp_2-a.mstat -a2mdots 0 SAM: sortseq v3.1b (February 24, 1999) compiled 04/18/00_11:44:38 Reading alignment file tmp_2-a.mult (29 sequences, 310 columns) as A2M alignment. Reading scores from file tmp_2-a.mstat Writing sequence output to tmp_2-a-sort.a2m. @@@@ cat m0.a2m tmp_2-a-sort.a2m > tmp_2-a.train.seq @@@@ cat m0.cst tmp_2-a-md.cst > tmp_2-a.train.cst # About to count sequences in tmp_2-a.train.seq # num_seqs=30 nll_thresh=-21.6394065571694 frac_id=0.999410959515382 @@@@ uniqueseq thinned_tmp -alignfile tmp_2-a.train.seq -a protein \ -percent_id 0.999410959515382 -a2mdots 0 SAM: uniqueseq v3.1b (February 24, 1999) compiled 04/18/00_11:44:50 Reading alignment file tmp_2-a.train.seq (30 sequences, 310 columns) as A2M alignment. Writing sequence output to thinned_tmp.a2m. Dropping 1 (of 30) duplicate sequences with differing IDs 29 sequences left after dropping 1 of 30 sequences. @@@@ modelfromalign junk_for_weights \ -alignfile tmp_2-a.train.seq \ -aweight_bits 0.7\ -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1 \ -aweight_method 1\ -aweight_exponent 10 SAM: modelfromalign v3.1b (February 24, 1999) compiled 04/18/00_11:44:13 Reading alignment file tmp_2-a.train.seq (29 sequences, 310 columns) as A2M alignment. @@@@ buildmodel tmp_2-b -insert tmp_2-a.mod\ -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1\ -modellength 0 \ -internal_weight 0 \ -anneal_length 2 \ -many_files 1 \ -Nseq 20000\ -nsurgery 0 \ -fracinsert 0.2 \ -cutinsert 0.25 -cutmatch 0.2 -initial_noise 0.0001 -anneal_noise 0.000001 \ -internal_weight 0 \ -train tmp_2-a.train.seq \ -nmodels 1\ -insert /projects/compbio/lib/stiff-gap5.regularizer -sequence_weights tmp_2-a.w -constraints tmp_2-a.train.cst Reading parameter file tmp_2-a.mod tmp_2-a.mod(23): Reading MODEL -- Model from alignment file /projects/compbio/experiments/casp4/t87/T0087.t2k_1.a2m Command line option(48): internal_weight has already been set. Using 0. Reading parameter file /projects/compbio/lib/stiff-gap5.regularizer /projects/compbio/lib/stiff-gap5.regularizer(1): Reading REGULARIZER: gap5. stiff regularizer, gaps averge 5, matches average 10 SAM: buildmodel v3.1b (February 24, 1999) compiled 04/18/00_11:43:59 -866.98 -51.25 -174.20 151.22 12 0 311 @@@@ hmmscore tmp_2-b -i tmp_2-b.mod -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1\ -dpstyle 0 \ -subtract_null 4 -sort 2 \ -db_size 499925 \ -db m0.a2m -db tmp_2-a.sel \ -select_score 4 \ -select_seq 4 \ -simple_threshold -25.6394065571694 -Emax 0.0002 \ -select_mdalign 4 -select_md 4 \ -adpstyle 1 \ -Motifcutoff 0.0645161290322581 \ -align_short 5 -mdEmax 0.0002 -constraints tmp_2-a.train.cst -constraints_out /projects/compbio/experiments/casp4/t87/T0087.t2k_2.cst Reading parameter file tmp_2-b.mod tmp_2-b.mod(20): Reading MODEL -- Final model for run tmp_2-b SAM: hmmscore v3.1b (February 24, 1999) compiled 04/18/00_11:44:03 Calculating Distances (. = 1000 sequences) . Average NLL-Simple NULL score: -203.933304 Database has 30 sequences with 12274 residues. @@@@ cp -f tmp_2-b.mult /projects/compbio/experiments/casp4/t87/T0087.t2k_2.a2m # About to count sequences in prefilter_3.fa @@@@ modelfromalign tmp_3-a -alignfile /projects/compbio/experiments/casp4/t87/T0087.t2k_2.a2m \ -insert /projects/compbio/lib/stiff-gap5.regularizer \ -aweight_bits 0.6\ -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1 \ -aweight_method 1\ -aweight_exponent 10 -constraints /projects/compbio/experiments/casp4/t87/T0087.t2k_2.cst -constraints_out tmp_3-a.cst Reading parameter file /projects/compbio/lib/stiff-gap5.regularizer /projects/compbio/lib/stiff-gap5.regularizer(1): Reading REGULARIZER: gap5. stiff regularizer, gaps averge 5, matches average 10 SAM: modelfromalign v3.1b (February 24, 1999) compiled 04/18/00_11:44:13 Reading alignment file /projects/compbio/experiments/casp4/t87/T0087.t2k_2.a2m (30 sequences, 310 columns) as A2M alignment. @@@@ hmmscore tmp_3-a -i tmp_3-a.mod -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1\ -dpstyle 0 \ -subtract_null 4 -sort 2 \ -db_size 499925 \ -simple_threshold -24.029968643135 -Emax 0.001 \ -adpstyle 1 \ -align_short 0 -mdEmax 0.001\ -select_score 4 \ -select_seq 4 \ -select_mdalign 4 -select_md 4 -Motifcutoff 0.0645161290322581 -db prefilter_3.fa -constraints tmp_3-a.cst -constraints_out tmp_3-a-md.cst Reading parameter file tmp_3-a.mod tmp_3-a.mod(23): Reading MODEL -- Model from alignment file /projects/compbio/experiments/casp4/t87/T0087.t2k_2.a2m SAM: hmmscore v3.1b (February 24, 1999) compiled 04/18/00_11:44:03 Calculating Distances (. = 1000 sequences) . Average NLL-Simple NULL score: -111.812042 Database has 53 sequences with 23679 residues. @@@@ sortseq tmp_3-a-sort -alignfile tmp_3-a.mult \ -NLLfile tmp_3-a.mstat -a2mdots 0 SAM: sortseq v3.1b (February 24, 1999) compiled 04/18/00_11:44:38 Reading alignment file tmp_3-a.mult (35 sequences, 310 columns) as A2M alignment. Reading scores from file tmp_3-a.mstat Writing sequence output to tmp_3-a-sort.a2m. @@@@ cat m0.a2m tmp_3-a-sort.a2m > tmp_3-a.train.seq @@@@ cat m0.cst tmp_3-a-md.cst > tmp_3-a.train.cst # About to count sequences in tmp_3-a.train.seq # num_seqs=36 nll_thresh=-20.029968643135 frac_id=0.996944616831446 @@@@ uniqueseq thinned_tmp -alignfile tmp_3-a.train.seq -a protein \ -percent_id 0.996944616831446 -a2mdots 0 SAM: uniqueseq v3.1b (February 24, 1999) compiled 04/18/00_11:44:50 Reading alignment file tmp_3-a.train.seq (36 sequences, 310 columns) as A2M alignment. Writing sequence output to thinned_tmp.a2m. Dropping 1 (of 36) duplicate sequences with differing IDs 35 sequences left after dropping 1 of 36 sequences. @@@@ modelfromalign junk_for_weights \ -alignfile tmp_3-a.train.seq \ -aweight_bits 0.6\ -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1 \ -aweight_method 1\ -aweight_exponent 10 SAM: modelfromalign v3.1b (February 24, 1999) compiled 04/18/00_11:44:13 Reading alignment file tmp_3-a.train.seq (35 sequences, 310 columns) as A2M alignment. @@@@ buildmodel tmp_3-b -insert tmp_3-a.mod\ -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1\ -modellength 0 \ -internal_weight 0 \ -anneal_length 2 \ -many_files 1 \ -Nseq 20000\ -nsurgery 0 \ -fracinsert 0.2 \ -cutinsert 0.25 -cutmatch 0.2 -initial_noise 0.0001 -anneal_noise 0.000001 \ -internal_weight 0 \ -train tmp_3-a.train.seq \ -nmodels 1\ -insert /projects/compbio/lib/stiff-gap5.regularizer -sequence_weights tmp_3-a.w -constraints tmp_3-a.train.cst Reading parameter file tmp_3-a.mod tmp_3-a.mod(23): Reading MODEL -- Model from alignment file /projects/compbio/experiments/casp4/t87/T0087.t2k_2.a2m Command line option(48): internal_weight has already been set. Using 0. Reading parameter file /projects/compbio/lib/stiff-gap5.regularizer /projects/compbio/lib/stiff-gap5.regularizer(1): Reading REGULARIZER: gap5. stiff regularizer, gaps averge 5, matches average 10 SAM: buildmodel v3.1b (February 24, 1999) compiled 04/18/00_11:43:59 -867.30 -78.68 -162.73 136.00 8 0 311 @@@@ hmmscore tmp_3-b -i tmp_3-b.mod -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1\ -dpstyle 0 \ -subtract_null 4 -sort 2 \ -db_size 499925 \ -db m0.a2m -db tmp_3-a.sel \ -select_score 4 \ -select_seq 4 \ -simple_threshold -24.029968643135 -Emax 0.001 \ -select_mdalign 4 -select_md 4 \ -adpstyle 1 \ -Motifcutoff 0.0645161290322581 \ -align_short 5 -mdEmax 0.001 -constraints tmp_3-a.train.cst -constraints_out /projects/compbio/experiments/casp4/t87/T0087.t2k_3.cst Reading parameter file tmp_3-b.mod tmp_3-b.mod(20): Reading MODEL -- Final model for run tmp_3-b SAM: hmmscore v3.1b (February 24, 1999) compiled 04/18/00_11:44:03 Calculating Distances (. = 1000 sequences) . Average NLL-Simple NULL score: -187.616760 Database has 36 sequences with 14502 residues. @@@@ cp -f tmp_3-b.mult /projects/compbio/experiments/casp4/t87/T0087.t2k_3.a2m # About to count sequences in prefilter_4.fa @@@@ modelfromalign tmp_4-a -alignfile /projects/compbio/experiments/casp4/t87/T0087.t2k_3.a2m \ -insert /projects/compbio/lib/fssp-trained.regularizer \ -aweight_bits 0.5\ -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1 \ -aweight_method 1\ -aweight_exponent 10 -constraints /projects/compbio/experiments/casp4/t87/T0087.t2k_3.cst -constraints_out tmp_4-a.cst Reading parameter file /projects/compbio/lib/fssp-trained.regularizer /projects/compbio/lib/fssp-trained.regularizer(1): Reading REGULARIZER: transitions from FSSP and pseudocounts for diverse weighted HSSP SAM: modelfromalign v3.1b (February 24, 1999) compiled 04/18/00_11:44:13 Reading alignment file /projects/compbio/experiments/casp4/t87/T0087.t2k_3.a2m (36 sequences, 310 columns) as A2M alignment. @@@@ hmmscore tmp_4-a -i tmp_4-a.mod -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1\ -dpstyle 0 \ -subtract_null 4 -sort 2 \ -db_size 499925 \ -simple_threshold -22.4205307226997 -Emax 0.005 \ -adpstyle 1 \ -align_short 0 -mdEmax 0.005\ -select_score 4 \ -select_seq 4 \ -select_mdalign 4 -select_md 4 -Motifcutoff 0.0645161290322581 -db prefilter_4.fa -constraints tmp_4-a.cst -constraints_out tmp_4-a-md.cst Reading parameter file tmp_4-a.mod tmp_4-a.mod(23): Reading MODEL -- Model from alignment file /projects/compbio/experiments/casp4/t87/T0087.t2k_3.a2m SAM: hmmscore v3.1b (February 24, 1999) compiled 04/18/00_11:44:03 Calculating Distances (. = 1000 sequences) . Average NLL-Simple NULL score: -20.490805 Database has 507 sequences with 186802 residues. @@@@ sortseq tmp_4-a-sort -alignfile tmp_4-a.mult \ -NLLfile tmp_4-a.mstat -a2mdots 0 SAM: sortseq v3.1b (February 24, 1999) compiled 04/18/00_11:44:38 Reading alignment file tmp_4-a.mult (39 sequences, 310 columns) as A2M alignment. Reading scores from file tmp_4-a.mstat Writing sequence output to tmp_4-a-sort.a2m. @@@@ cat m0.a2m tmp_4-a-sort.a2m > tmp_4-a.train.seq @@@@ cat m0.cst tmp_4-a-md.cst > tmp_4-a.train.cst # About to count sequences in tmp_4-a.train.seq # num_seqs=40 nll_thresh=-18.4205307226997 frac_id=0.992078913784903 @@@@ uniqueseq thinned_tmp -alignfile tmp_4-a.train.seq -a protein \ -percent_id 0.992078913784903 -a2mdots 0 SAM: uniqueseq v3.1b (February 24, 1999) compiled 04/18/00_11:44:50 Reading alignment file tmp_4-a.train.seq (40 sequences, 310 columns) as A2M alignment. Writing sequence output to thinned_tmp.a2m. Dropping 1 (of 40) duplicate sequences with differing IDs Dropping 1 (of 40) sequences with > 99.2% id 38 sequences left after dropping 2 of 40 sequences. @@@@ modelfromalign junk_for_weights \ -alignfile tmp_4-a.train.seq \ -aweight_bits 0.5\ -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1 \ -aweight_method 1\ -aweight_exponent 10 SAM: modelfromalign v3.1b (February 24, 1999) compiled 04/18/00_11:44:13 Reading alignment file tmp_4-a.train.seq (38 sequences, 310 columns) as A2M alignment. @@@@ buildmodel tmp_4-b -insert tmp_4-a.mod\ -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1\ -modellength 0 \ -internal_weight 0 \ -anneal_length 2 \ -many_files 1 \ -Nseq 20000\ -nsurgery 0 \ -fracinsert 0.2 \ -cutinsert 0.25 -cutmatch 0.2 -initial_noise 0.0001 -anneal_noise 0.000001 \ -internal_weight 0 \ -train tmp_4-a.train.seq \ -nmodels 1\ -insert /projects/compbio/lib/fssp-trained.regularizer -sequence_weights tmp_4-a.w -constraints tmp_4-a.train.cst Reading parameter file tmp_4-a.mod tmp_4-a.mod(23): Reading MODEL -- Model from alignment file /projects/compbio/experiments/casp4/t87/T0087.t2k_3.a2m Command line option(48): internal_weight has already been set. Using 0. Reading parameter file /projects/compbio/lib/fssp-trained.regularizer /projects/compbio/lib/fssp-trained.regularizer(1): Reading REGULARIZER: transitions from FSSP and pseudocounts for diverse weighted HSSP SAM: buildmodel v3.1b (February 24, 1999) compiled 04/18/00_11:43:59 -868.16 -54.30 -169.00 127.18 12 0 311 @@@@ hmmscore tmp_4-b -i tmp_4-b.mod -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1\ -dpstyle 0 \ -subtract_null 4 -sort 2 \ -db_size 499925 \ -db m0.a2m -db tmp_4-a.sel \ -select_score 4 \ -select_seq 4 \ -simple_threshold -22.4205307226997 -Emax 0.005 \ -select_mdalign 4 -select_md 4 \ -adpstyle 1 \ -Motifcutoff 0.0645161290322581 \ -align_short 5 -mdEmax 0.005 -constraints tmp_4-a.train.cst -constraints_out /projects/compbio/experiments/casp4/t87/T0087.t2k_4.cst Reading parameter file tmp_4-b.mod tmp_4-b.mod(20): Reading MODEL -- Final model for run tmp_4-b SAM: hmmscore v3.1b (February 24, 1999) compiled 04/18/00_11:44:03 Calculating Distances (. = 1000 sequences) . Average NLL-Simple NULL score: -181.703278 Database has 40 sequences with 16217 residues. @@@@ cp -f tmp_4-b.mult /projects/compbio/experiments/casp4/t87/T0087.t2k_4.a2m @@@@ modelfromalign tmp_5-a -alignfile /projects/compbio/experiments/casp4/t87/T0087.t2k_4.a2m \ -insert /projects/compbio/lib/fssp-trained.regularizer \ -aweight_bits 0.5\ -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1 \ -aweight_method 1\ -aweight_exponent 10 -constraints /projects/compbio/experiments/casp4/t87/T0087.t2k_4.cst -constraints_out tmp_5-a.cst Reading parameter file /projects/compbio/lib/fssp-trained.regularizer /projects/compbio/lib/fssp-trained.regularizer(1): Reading REGULARIZER: transitions from FSSP and pseudocounts for diverse weighted HSSP SAM: modelfromalign v3.1b (February 24, 1999) compiled 04/18/00_11:44:13 Reading alignment file /projects/compbio/experiments/casp4/t87/T0087.t2k_4.a2m (40 sequences, 310 columns) as A2M alignment. @@@@ hmmscore tmp_5-a -i tmp_5-a.mod -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1\ -dpstyle 0 \ -subtract_null 4 -sort 2 \ -db_size 499925 \ -simple_threshold -22.4205307226997 -Emax 0.005 \ -adpstyle 5 \ -align_short 0 -mdEmax 0.005\ -select_score 4 \ -select_seq 4 \ -select_mdalign 4 -select_md 4 -Motifcutoff 0.0645161290322581 -db prefilter_4.fa -constraints tmp_5-a.cst -constraints_out tmp_5-a-md.cst Reading parameter file tmp_5-a.mod tmp_5-a.mod(23): Reading MODEL -- Model from alignment file /projects/compbio/experiments/casp4/t87/T0087.t2k_4.a2m SAM: hmmscore v3.1b (February 24, 1999) compiled 04/18/00_11:44:03 Calculating Distances (. = 1000 sequences) . Average NLL-Simple NULL score: -21.352509 Database has 507 sequences with 186802 residues. @@@@ sortseq tmp_5-a-sort -alignfile tmp_5-a.mult \ -NLLfile tmp_5-a.mstat -a2mdots 0 SAM: sortseq v3.1b (February 24, 1999) compiled 04/18/00_11:44:38 Reading alignment file tmp_5-a.mult (39 sequences, 310 columns) as A2M alignment. Reading scores from file tmp_5-a.mstat Writing sequence output to tmp_5-a-sort.a2m. @@@@ cat m0.a2m tmp_5-a-sort.a2m > tmp_5-a.train.seq @@@@ cat m0.cst tmp_5-a-md.cst > tmp_5-a.train.cst @@@@ cp tmp_5-a.train.seq /projects/compbio/experiments/casp4/t87/T0087.t2k.a2m @@@@ cp tmp_5-a.train.cst /projects/compbio/experiments/casp4/t87/T0087.t2k.cst @@@@ rm -rf /projects/compbio/tmp/target2k-beta-3921 < /dev/null gzip -f T0087.t2k*.a2m prettyalign T0087.t2k.a2m.gz -m8 -i -n -L3333 > T0087.t2k.pa SAM: prettyalign v3.1b (February 24, 1999) compiled 04/18/00_11:44:15 gunzip -c T0087.t2k.a2m.gz > tmp.a2m a2m2html -a2m_in tmp.a2m -retrieve > T0087.t2k.pa.html SAM: /projects/compbio/bin/alpha/prettyalign v3.1b (February 24, 1999) compiled 04/18/00_11:44:15 rm tmp.a2m echo ReadNeuralNet /projects/compbio/usr/karplus/predict-2nd/testing/networks/overrep-2500-IDaa13-5-9-7-10-5-9-11-ehl2-seeded-stride-trained.net > tmp.script echo ReadA2M T0087.t2k.a2m.gz >> tmp.script echo PrintPrediction T0087.t2k.2d 3670-4530-6947 >> tmp.script echo PrintPredictionFasta T0087.t2k.2d.seq >> tmp.script echo PrintRDB T0087.t2k.2d.rdb >> tmp.script predict-2nd < tmp.script # command:# Neural network set to overrep-2500-IDaa13-5-9-7-10-5-9-11-ehl2-seeded-stride-trained.net # command:# Reading A2M format from T0087.t2k.a2m.gz # Using SequenceWeight EntropyWeight(1.4, 10) # Using regularizer /projects/compbio/lib/recode3.20comp for sequence weight regularizer. # T0087.t2k.a2m with 40 sequences, total weight= 27.6601 avg weight= 0.691503 clipped 46 iterations # AdjustWeights couldn't save exactly 1.4 bits/position, saving 0.94062 bits. Regularizing alignment for T0087.t2k.a2m.gz # After reading T0087.t2k.a2m.gz, have 310 columns in 1 chains # command:# Initializing Gain for FinalLayer # Initializing Gain for ordering_layer # Initializing Gain for SecondLayer # Initializing Gain for FirstLayer # Network initialization done # Printing prediction to T0087.t2k.2d # command:# Network initialization done # Printing prediction in FASTA format to T0087.t2k.2d.seq # command:# Network initialization done # Printing prediction to T0087.t2k.2d.rdb # command:rm tmp.script gunzip -f T0087.t2k.a2m.gz /projects/compbio/usr/karplus/src/phytree/phytree -o -i -r flat T0087.t2k T0087.t2k.a2m /projects/compbio/lib/recode2.20comp Prior library /projects/compbio/lib/recode2.20comp read. Reading alignment file T0087.t2k.a2m (40 sequences) as A2M alignment. Alignment T0087.t2k.a2m read. Adding sequences:0 10 20 30 One dot for every 10 merges: .... Merges done. CPU = user 0: 0:43 system 0: 0: 1 gzip -f T0087.t2k.a2m gzip -f T0087.t2k_sorted.a2m rm T0087.t2k.phytrace T0087.t2k.tree_weight prettyalign T0087.t2k_sorted.a2m.gz -m8 -i -n -L3333 > T0087.t2k_sorted.pa SAM: prettyalign v3.1b (February 24, 1999) compiled 04/18/00_11:44:15 /projects/compbio/usr/karplus/src/phytree/dtree T0087.t2k.tree make[1]: Leaving directory `/auto/projects/compbio/experiments/casp4/t87' /projects/compbio/experiments/models.97/scripts99/score-target -alignment T0087.t2k.a2m.gz -build w0.5 \ -db /projects/compbio/data/pdb/all-protein \ -Emax 10.0 \ -viterbi 0 -sw_score 2 \ -output T0087.t2k-w0.5-pdb.rdb PATH=.:/projects/compbio/experiments/models.97/scripts99:/projects/compbio/bin:/projects/compbio/bin/alpha:/projects/compbio/bin/hmmscripts:/projects/compbio/bin/scripts:/sbin:/usr/sbin:/bin:/usr/bin:/usr/local/gnu/bin:/usr/local/share/request/bin:/usr/local/bin @@@@ mkdir /projects/compbio/tmp/score-target-18235 @@@@ mkdir /projects/compbio/tmp/score-target-18235/for-scorescript @@@@ /projects/compbio/experiments/models.97/scripts99/score-mods-vs-seqs.pl -root_tmp /projects/compbio/tmp/score-target-18235/for-scorescript -a2msfile /projects/compbio/tmp/score-target-18235/score-target.a2m \ -buildscript /projects/compbio/bin/scripts/w0.5 -seqfile /projects/compbio/data/pdb/all-protein -Emax 10.0 -viterbi 0 \ -sw_score 2 -output /projects/compbio/tmp/score-target-18235/score-target.op @@@@ chgrp protein /projects/compbio/tmp/score-target-18235/for-scorescript/split-tmp @@@@ chgrp protein /projects/compbio/tmp/score-target-18235/for-scorescript/split-tmp @@@@ /projects/compbio/bin/scripts/w0.5 /projects/compbio/experiments/casp4/t87/T0087.t2k.a2m.gz /projects/compbio/tmp/score-target-18235/for-scorescript/split-tmp/beta-30964/T0087.t2k.mod Reading /projects/compbio/bin/scripts/sam-t99.conf @@@@ chgrp protein /projects/compbio/tmp/tmp-build-weighted-model-beta-25229 @@@@ uniqueseq /projects/compbio/tmp/tmp-build-weighted-model-beta-25229/tmp -alignfile /projects/compbio/experiments/casp4/t87/T0087.t2k.a2m.gz \ -a protein -percent_id 0.8 SAM: uniqueseq v3.1b (February 24, 1999) compiled 04/18/00_11:44:50 Reading alignment file /projects/compbio/experiments/casp4/t87/T0087.t2k.a2m.gz (40 sequences, 310 columns) as A2M alignment. Writing sequence output to /projects/compbio/tmp/tmp-build-weighted-model-beta-25229/tmp.a2m. Dropping 6 (of 40) sequences with > 80.0% id 34 sequences left after dropping 6 of 40 sequences. @@@@ modelfromalign /projects/compbio/tmp/tmp-build-weighted-model-beta-25229/tmp -alignfile /projects/compbio/tmp/tmp-build-weighted-model-beta-25229/tmp.a2m \ -insert /projects/compbio/lib/fssp-trained.regularizer \ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -prior_library /projects/compbio/lib/recode3.20comp \ -binary_output 1 -a protein -aweight_method 1 -aweight_bits 0.5 -aweight_exponent 10 Reading parameter file /projects/compbio/lib/fssp-trained.regularizer /projects/compbio/lib/fssp-trained.regularizer(1): Reading REGULARIZER: transitions from FSSP and pseudocounts for diverse weighted HSSP SAM: modelfromalign v3.1b (February 24, 1999) compiled 04/18/00_11:44:13 Reading alignment file /projects/compbio/tmp/tmp-build-weighted-model-beta-25229/tmp.a2m (34 sequences, 310 columns) as A2M alignment. @@@@ cp -f /projects/compbio/tmp/tmp-build-weighted-model-beta-25229/tmp.mod /projects/compbio/tmp/score-target-18235/for-scorescript/split-tmp/beta-30964/T0087.t2k.mod @@@@ rm -rf /projects/compbio/tmp/tmp-build-weighted-model-beta-25229 80.0% id 7 sequences left after dropping 235 of 242 sequences. @@@@ modelfromalign /projects/compbio/tmp/tmp-build-weighted-model-beta-18828/tmp -alignfile /projects/compbio/tmp/tmp-build-weighted-model-beta-18828/tmp.a2m \ -insert /projects/compbio/lib/fssp-trained.regularizer \ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -prior_library /projects/compbio/lib/recode3.20comp \ -binary_output 1 -a protein -aweight_method 1 -aweight_bits 0.5 -aweight_exponent 10 Reading parameter file /projects/compbio/lib/fssp-trained.regularizer /projects/compbio/lib/fssp-trained.regularizer(1): Reading REGULARIZER: transitions from FSSP and pseudocounts for diverse weighted HSSP SAM: modelfromalign v3.1b (February 24, 1999) compiled 04/18/00_11:44:13 Reading alignment file /projects/compbio/tmp/tmp-build-weighted-model-beta-18828/tmp.a2m (7 sequences, 388 columns) as A2M alignment. @@@@ cp -f /projects/compbio/tmp/tmp-build-weighted-model-beta-18828/tmp.mod /projects/compbio/experiments/models.97/pdb/3n/3nn9/nostruct-align/3nn9.t99-w0.5.mod @@@@ rm -rf /projects/compbio/tmp/tmp-build-weighted-model-beta-18828 80.0% id 110 sequences left after dropping 154 of 264 sequences. @@@@ modelfromalign /projects/compbio/tmp/tmp-build-weighted-model-beta-8238/tmp -alignfile /projects/compbio/tmp/tmp-build-weighted-model-beta-8238/tmp.a2m \ -insert /projects/compbio/lib/fssp-trained.regularizer \ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -prior_library /projects/compbio/lib/recode3.20comp \ -binary_output 1 -a protein -aweight_method 1 -aweight_bits 0.5 -aweight_exponent 10 Reading parameter file /projects/compbio/lib/fssp-trained.regularizer /projects/compbio/lib/fssp-trained.regularizer(1): Reading REGULARIZER: transitions from FSSP and pseudocounts for diverse weighted HSSP SAM: modelfromalign v3.1b (February 24, 1999) compiled 04/18/00_11:44:13 Reading alignment file /projects/compbio/tmp/tmp-build-weighted-model-beta-8238/tmp.a2m (110 sequences, 201 columns) as A2M alignment. @@@@ cp -f /projects/compbio/tmp/tmp-build-weighted-model-beta-8238/tmp.mod /projects/compbio/experiments/models.97/pdb/3a/3aig/nostruct-align/3aig.t99-w0.5.mod @@@@ rm -rf /projects/compbio/tmp/tmp-build-weighted-model-beta-8238 80.0% id 40 sequences left after dropping 58 of 98 sequences. @@@@ modelfromalign /projects/compbio/tmp/tmp-build-weighted-model-beta-28024/tmp -alignfile /projects/compbio/tmp/tmp-build-weighted-model-beta-28024/tmp.a2m \ -insert /projects/compbio/lib/fssp-trained.regularizer \ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -prior_library /projects/compbio/lib/recode3.20comp \ -binary_output 1 -a protein -aweight_method 1 -aweight_bits 0.5 -aweight_exponent 10 Reading parameter file /projects/compbio/lib/fssp-trained.regularizer /projects/compbio/lib/fssp-trained.regularizer(1): Reading REGULARIZER: transitions from FSSP and pseudocounts for diverse weighted HSSP SAM: modelfromalign v3.1b (February 24, 1999) compiled 04/18/00_11:44:13 Reading alignment file /projects/compbio/tmp/tmp-build-weighted-model-beta-28024/tmp.a2m (40 sequences, 282 columns) as A2M alignment. @@@@ cp -f /projects/compbio/tmp/tmp-build-weighted-model-beta-28024/tmp.mod /projects/compbio/experiments/models.97/pdb/8p/8prkB/nostruct-align/8prkB.t99-w0.5.mod @@@@ rm -rf /projects/compbio/tmp/tmp-build-weighted-model-beta-28024 tmp.script echo ReadA2M T0087.t2k.a2m.gz >> tmp.script echo PrintPrediction T0087.t2k.2d 3670-4530-6947 >> tmp.script echo PrintPredictionFasta T0087.t2k.2d.seq >> tmp.script echo PrintRDB T0087.t2k.2d.rdb >> tmp.script predict-2nd < tmp.script # command:# Neural network set to overrep-2500-IDaa13-5-9-7-10-5-9-11-ehl2-seeded2-stride-trained.net # command:# Reading A2M format from T0087.t2k.a2m.gz # Using SequenceWeight EntropyWeight(1.4, 10) # Using regularizer /projects/compbio/lib/recode3.20comp for sequence weight regularizer. # T0087.t2k.a2m with 40 sequences, total weight= 27.6601 avg weight= 0.691503 clipped 46 iterations # AdjustWeights couldn't save exactly 1.4 bits/position, saving 0.94062 bits. Regularizing alignment for T0087.t2k.a2m.gz # After reading T0087.t2k.a2m.gz, have 310 columns in 1 chains # command:# Initializing Gain for FinalLayer # Initializing Gain for ordering_layer # Initializing Gain for SecondLayer # Initializing Gain for FirstLayer # Network initialization done # Printing prediction to T0087.t2k.2d # command:# Network initialization done # Printing prediction in FASTA format to T0087.t2k.2d.seq # command:# Network initialization done # Printing prediction to T0087.t2k.2d.rdb # command:rm tmp.script make[1]: `T0087.t2k.tree' is up to date. make[1]: `T0087.t2k_sorted.pa' is up to date. make[1]: `T0087.t2k.tree-unroot.ps' is up to date. make[1]: Leaving directory `/auto/projects/compbio/experiments/casp4/t87' echo ReadNeuralNet /projects/compbio/usr/karplus/predict-2nd/testing/networks/overrep-2500-IDaa13-5-9-7-10-5-10-11-ehl2-seeded4-stride-trained.net > tmp.script echo ReadA2M T0087.t2k.a2m.gz >> tmp.script echo PrintPrediction T0087.t2k.2d 3670-4530-6947 >> tmp.script echo PrintPredictionFasta T0087.t2k.2d.seq >> tmp.script echo PrintRDB T0087.t2k.2d.rdb >> tmp.script predict-2nd < tmp.script # command:# Neural network set to overrep-2500-IDaa13-5-9-7-10-5-10-11-ehl2-seeded4-stride-trained.net # command:# Reading A2M format from T0087.t2k.a2m.gz # Using SequenceWeight EntropyWeight(1.4, 10) # Using regularizer /projects/compbio/lib/recode3.20comp for sequence weight regularizer. # T0087.t2k.a2m with 40 sequences, total weight= 27.6601 avg weight= 0.691503 clipped 46 iterations # AdjustWeights couldn't save exactly 1.4 bits/position, saving 0.94062 bits. Regularizing alignment for T0087.t2k.a2m.gz # After reading T0087.t2k.a2m.gz, have 310 columns in 1 chains # command:# Initializing Gain for FinalLayer # Initializing Gain for ordering_layer # Initializing Gain for SecondLayer # Initializing Gain for FirstLayer # Network initialization done # Printing prediction to T0087.t2k.2d # command:# Network initialization done # Printing prediction in FASTA format to T0087.t2k.2d.seq # command:# Network initialization done # Printing prediction to T0087.t2k.2d.rdb # command:rm tmp.script make: Nothing to be done for `T0087.t2k.2d.rdb'. make: Nothing to be done for `T0087.t2k.2d.seq'. echo ReadNeuralNet /projects/compbio/usr/karplus/predict-2nd/testing/networks/overrep-2500-IDaa13-5-10-7-10-5-10-11-ehl2-seeded9-stride-trained.net > tmp.script echo ReadA2M T0087.t2k.a2m.gz >> tmp.script echo PrintPrediction T0087.t2k.2d 3670-4530-6947 >> tmp.script echo PrintPredictionFasta T0087.t2k.2d.seq >> tmp.script echo PrintRDB T0087.t2k.2d.rdb >> tmp.script predict-2nd < tmp.script # command:# Neural network set to overrep-2500-IDaa13-5-10-7-10-5-10-11-ehl2-seeded9-stride-trained.net # command:# Reading A2M format from T0087.t2k.a2m.gz # Using SequenceWeight EntropyWeight(1.4, 10) # Using regularizer /projects/compbio/lib/recode3.20comp for sequence weight regularizer. # T0087.t2k.a2m with 40 sequences, total weight= 27.6601 avg weight= 0.691503 clipped 46 iterations # AdjustWeights couldn't save exactly 1.4 bits/position, saving 0.94062 bits. Regularizing alignment for T0087.t2k.a2m.gz # After reading T0087.t2k.a2m.gz, have 310 columns in 1 chains # command:# Initializing Gain for FinalLayer # Initializing Gain for ordering_layer # Initializing Gain for SecondLayer # Initializing Gain for FirstLayer # Network initialization done # Printing prediction to T0087.t2k.2d # command:# Network initialization done # Printing prediction in FASTA format to T0087.t2k.2d.seq # command:# Network initialization done # Printing prediction to T0087.t2k.2d.rdb # command:rm tmp.script make: `T0087.t2k.2d' is up to date.