CASP4 Target T0124

1. Protein Name

Phospholipase C beta C-terminus

2. Organism Name

turkey (Meleagris gallopavo)

3. Number of amino acids (approx)

242

4. Accession number

U49431

5. Sequence Database

Genbank

6. Amino acid sequence

NMKEVTQLPE PQTASLAELQ QMKLFLKLLK KQEKELKELE RKGSKRREEL LQKYSVLFLE
PVYPRGLDSQ VVELKERLEM ELIHLGEEYH DGIRRRKEQH ATEQTAKITE LAREKQIAEL
KALKESSESN IKDIKKKLEA KRLDRIQVMM RSTSDKAAQE RLKKEINNSH IQEVVQTIKL
LTEKTARYQQ KLEEKQAENL RAIQEKEGQL QQEAVAEYEE KLKTLTVEVQ EMVKNYMKEV
FP

7. Additional Information

Unlike other Phospholipase C (PLC) isozymes, PLC beta enzymes contain a
region of sequence homology of ~250 amino acids near the C-terminus.
This region is involved in activation of the lipase activity of PLC beta
by GTP-bound G alpha q subunits and in turn acts as a GTPase Activating
Protein (GAP) for G alpha q. This C-terminal 250 amino acid domain contains
no obvious homology to known structures. I have solved the
structure of this C-terminal domain representing amino acids
880-1154 of a PLC beta isolated from turkey erythrocytes.
Initially crystals from this construct diffracted poorly (~7 A),
so a region spanning amino acids 946-978 was deleted. This
region is highly variable among PLCs, is protease-sensitive
and is a site for serine phosphorylation. The crystals were grown
between pH 5.7 to 6.3 and are triclinic. I would like to withhold
information about the oligomerization state at present as I
feel this is one of the interesting aspects of this structure.

8. Homologous Sequence of known structure

no

9. Current state of the experimental work

This structure was solved by MAD using crystals containing
selenomethionine-labeled protein. I have refined to a resolution
of 2.4 A, with an R and Rfree of 25.4 and 29.3 respectively. Little
more will be done with regard to further refinement as there are no
outliers in Ramachandran phi/psi space and the geometry is well
within what is expected for a 2.4 A structure.

10. Interpretable map?

yes

11. Estimated date of chain tracing completion

Done

12. Estimated date of public release of structure

September 2000

13. Name

Unavailable until after public release of structure

Related Files

Template PDB file