CASP4 Target T0122

1. Protein Name

Tryptophan Synthase alpha subunit

2. Organism Name

Pyrococcus furiosus

3. Number of amino acids (approx)

248

4. Accession number

5. Sequence Database

not available

6. Amino acid sequence

MFKDGSLIPY LTAGDPDKQS TLNFLLALDE YAGAIELGIP FSDPIADGKT IQESHYRALK
NGFKLREAFW IVKEFRRHSS TPIVLMTYYN PIYRAGVRNF LAEAKASGVD GILVVDLPVF
HAKEFTEIAR EEGIKTVFLA APNTPDERLK VIDDMTTGFV YLVSLYGTTG AREEIPKTAY
DLLRRAKRIC RNKVAVGFGV SKREHVVSLL KEGANGVVVG SALVKIIGEK GREATEFLKK
KVEELLGI

7. Additional Information

In 1988 (1BKS; Hyde et al.) the three dimensional structure of the tryptophan synthase alapha2beta2 complex from Salmonella typhimurium has been determined by X-ray analysis. However, the structure of the alapha or beta2 subunit alone has not yet been determined. We picked up alapha-subunit from hyperthermophile, Pyrococcus furiosus
Because the sequence homologue of both proteins was 31.5 %, molecular replacement method (MR) using the structure of alapha-subunit in the alapha2beta2 complex of tryptophan synthase from S. typhimurium (1BKS) were tried using AMoRe. But all attempts to refine the solution failed. Therefore, the structure of alpha-subunit from P. furisus was solved by the method of multiple isomorphous replacement (MIR) using two derivatives; Pt and Hg.
Recently, we could determine the structure of alapha subunit from P. furiosus at a resolution of 2.0 A(free R = 24.9, R = 19.8).

8. Homologous Sequence of known structure

yes

9. Current state of the experimental work

Recently, we could determine the structure of alpha subunit from P. furiosus at a resolution of 2.0 A (free R = 24.9, R = 19.8).

10. Interpretable map?

yes

11. Estimated date of chain tracing completion

already determined

12. Estimated date of public release of structure

April, 2001

13. Name

Unavailable until after public release of structure

Related Files

Template PDB file