make -k T0121-1-230-blast.rdb T0121-1-230-double-blast.rdb make[1]: Entering directory `/auto/projects/compbio/experiments/casp4/t121/t121-1-230' /projects/compbio/experiments/casp4/scripts/single-blast -q T0121-1-230.seq -interdb /projects/compbio/data/nrp/nr -db /projects/compbio/data/pdb/all-protein > T0121-1-230-blast.rdb starting T0121 with E=10 /projects/compbio/experiments/casp4/scripts/double-blast -q T0121-1-230.seq -interdb /projects/compbio/data/nrp/nr -db /projects/compbio/data/pdb/all-protein > T0121-1-230-double-blast.rdb starting T0121 with E=0.000050 EXIT CODE 26 T0121 T0121 blasting: /bin/sh: 24375 Terminated make[1]: *** [T0121-1-230-double-blast.rdb] Error 143 make[1]: Leaving directory `/auto/projects/compbio/experiments/casp4/t121/t121-1-230' make: [T0121-1-230.blast-all] Error 2 (ignored) make -k T0121-1-230.t2k.a2m.gz \ T0121-1-230.t2k.pa \ T0121-1-230.t2k.pa.html \ T0121-1-230.t2k.2d \ T0121-1-230.t2k.tree \ T0121-1-230.t2k_sorted.pa \ T0121-1-230.t2k.tree.ps \ T0121-1-230.t2k.tree-unroot.ps make[1]: Entering directory `/auto/projects/compbio/experiments/casp4/t121/t121-1-230' /projects/compbio/experiments/models.97/scripts2k/target2k -out T0121-1-230.t2k \ -seed T0121-1-230.seq \ -family \ -thresholds 1e-44,1e-40,1e-25 \ -prefilter_thresholds 1e-44,1e-40,1e-25 \ -all @@@@ chgrp protein /projects/compbio/tmp/target2k-eclipse-24420 @@@@ cp /projects/compbio/experiments/casp4/t121/t121-1-230/T0121-1-230.seq m0.a2m @@@@ checkseq foo -alphabet protein -db m0.a2m > init.check SAM: checkseq v3.2 (July 31, 2000) compiled 07/31/00_14:08:52 # About to count sequences in /projects/compbio/data/nrp/nr /projects/compbio/experiments/models.97/scripts2k/target2k: E-value thresholds used are prefilter: 1e-44, actual: 1e-44 prefilter: 1e-40, actual: 1e-40 prefilter: 1e-25, actual: 1e-25 /projects/compbio/experiments/models.97/scripts2k/target2k: aweight_bits are 0.8, 0.7, 0.5 /projects/compbio/experiments/models.97/scripts2k/target2k: db_size= 551100 reverse_diff= 4 /projects/compbio/experiments/models.97/scripts2k/target2k: blast_max_report= 10000 /projects/compbio/experiments/models.97/scripts2k/target2k: db=/projects/compbio/data/nrp/nr /projects/compbio/experiments/models.97/scripts2k/target2k: aweight_method=1 aweight_exponent=10 /projects/compbio/experiments/models.97/scripts2k/target2k: force_seed=1 constraints=1 /projects/compbio/experiments/models.97/scripts2k/target2k: jump_in=0.2 jump_out=1 fimstrength=1 fimtrans=-1 @@@@ blast-prefilter -out prefilter -seed m0.a2m -prefilter_thresholds 1e-44,1e-40,1e-25 -db /projects/compbio/data/nrp/nr -blast_max_report 10000 @@@@ chgrp protein /projects/compbio/tmp/blast-prefilter-eclipse-24475 /projects/compbio/experiments/models.97/scripts2k/blast-prefilter: E-value thresholds used are For /projects/compbio/tmp/target2k-eclipse-24420/prefilter_1.fa, 1e-44 For /projects/compbio/tmp/target2k-eclipse-24420/prefilter_2.fa, 1e-40 For /projects/compbio/tmp/target2k-eclipse-24420/prefilter_3.fa, 1e-25 /projects/compbio/experiments/models.97/scripts2k/blast-prefilter: db=/projects/compbio/data/nrp/nr @@@@ uniqueseq init -alignfile /projects/compbio/tmp/target2k-eclipse-24420/m0.a2m -a protein -a2mdots 0 SAM: uniqueseq v3.2 (July 31, 2000) compiled 07/31/00_14:08:59 Reading alignment file /projects/compbio/tmp/target2k-eclipse-24420/m0.a2m (1 sequences, 230 columns) as A2M alignment. Writing sequence output to init.a2m. No sequences have been dropped. @@@@ /projects/compbio/bin/wu-blastp /projects/compbio/data/nrp/nr 1.fasta E=1e-25 V=10000 B=0 -gi > 1.fasta-blast.out Searched 551100 sequences # parsing wu-blastp output @@@@ rm -f 1.fasta < /dev/null @@@@ rm -f 1.fasta-blast.out < /dev/null # Extracting hits in FASTA format from /projects/compbio/data/nrp/nr @@@@ rm -rf /projects/compbio/tmp/blast-prefilter-eclipse-24475 < /dev/null @@@@ modelfromalign unused -alignfile m0.a2m \ -constraints_from_align 1 -constraints_out m0.cst SAM: modelfromalign v3.2 (July 31, 2000) compiled 07/31/00_14:08:27 Reading alignment file m0.a2m (1 sequences, 230 columns) as A2M alignment. # About to count sequences in m0.a2m # About to count sequences in prefilter_1.fa @@@@ modelfromalign tmp_1-a -alignfile m0.a2m \ -insert /projects/compbio/lib/gap1.5.regularizer \ -aweight_bits 0.8\ -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1 \ -aweight_method 1\ -aweight_exponent 10 -constraints m0.cst -constraints_out tmp_1-a.cst Reading parameter file /projects/compbio/lib/gap1.5.regularizer /projects/compbio/lib/gap1.5.regularizer(1): Reading REGULARIZER: gap1.5. Weak regularizer, gaps average 1.5, matches average 10 SAM: modelfromalign v3.2 (July 31, 2000) compiled 07/31/00_14:08:27 Reading alignment file m0.a2m (1 sequences, 230 columns) as A2M alignment. @@@@ hmmscore tmp_1-a -i tmp_1-a.mod -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1\ -dpstyle 0 \ -subtract_null 4 -sort 2 \ -db_size 551100 \ -simple_threshold -118.533415651609 -Emax 1e-44 \ -adpstyle 1 \ -align_short 0 -mdEmax 1e-44\ -select_score 4 \ -select_seq 4 \ -select_mdalign 4 -select_md 4 -Motifcutoff 0.0869565217391304 -db prefilter_1.fa -constraints tmp_1-a.cst -constraints_out tmp_1-a-md.cst Reading parameter file tmp_1-a.mod tmp_1-a.mod(21): Reading MODEL -- Model from alignment file m0.a2m SAM: hmmscore v3.2 (July 31, 2000) compiled 08/04/00_11:31:33 Calculating Distances (. = 1000 sequences) sh: 24464 Floating exception *** Error: /projects/compbio/experiments/models.97/scripts2k/target2k: hmmscore to select sequences failed make[1]: *** [T0121-1-230.t2k.a2m.gz] Error 2 make[1]: Target `T0121-1-230.t2k.pa' not remade because of errors. make[1]: Target `T0121-1-230.t2k.pa.html' not remade because of errors. make[1]: Target `T0121-1-230.t2k.2d' not remade because of errors. make[1]: Target `T0121-1-230.t2k.tree' not remade because of errors. make[1]: Target `T0121-1-230.t2k_sorted.pa' not remade because of errors. make[1]: Target `T0121-1-230.t2k.tree.ps' not remade because of errors. make[1]: Target `T0121-1-230.t2k.tree-unroot.ps' not remade because of errors. make[1]: Leaving directory `/auto/projects/compbio/experiments/casp4/t121/t121-1-230' make: [T0121-1-230.align] Error 2 (ignored) /projects/compbio/experiments/models.97/scripts2k/target2k -out T0121-1-230.t2k \ -seed T0121-1-230.seq \ -family \ -thresholds 1e-44,1e-40,1e-25 \ -prefilter_thresholds 1e-44,1e-40,1e-25 \ -all @@@@ chgrp protein /projects/compbio/tmp/target2k-eclipse-24544 @@@@ cp /projects/compbio/experiments/casp4/t121/t121-1-230/T0121-1-230.seq m0.a2m @@@@ checkseq foo -alphabet protein -db m0.a2m > init.check SAM: checkseq v3.2 (July 31, 2000) compiled 07/31/00_14:08:52 # About to count sequences in /projects/compbio/data/nrp/nr /projects/compbio/experiments/models.97/scripts2k/target2k: E-value thresholds used are prefilter: 1e-44, actual: 1e-44 prefilter: 1e-40, actual: 1e-40 prefilter: 1e-25, actual: 1e-25 /projects/compbio/experiments/models.97/scripts2k/target2k: aweight_bits are 0.8, 0.7, 0.5 /projects/compbio/experiments/models.97/scripts2k/target2k: db_size= 551100 reverse_diff= 4 /projects/compbio/experiments/models.97/scripts2k/target2k: blast_max_report= 10000 /projects/compbio/experiments/models.97/scripts2k/target2k: db=/projects/compbio/data/nrp/nr /projects/compbio/experiments/models.97/scripts2k/target2k: aweight_method=1 aweight_exponent=10 /projects/compbio/experiments/models.97/scripts2k/target2k: force_seed=1 constraints=1 /projects/compbio/experiments/models.97/scripts2k/target2k: jump_in=0.2 jump_out=1 fimstrength=1 fimtrans=-1 @@@@ blast-prefilter -out prefilter -seed m0.a2m -prefilter_thresholds 1e-44,1e-40,1e-25 -db /projects/compbio/data/nrp/nr -blast_max_report 10000 @@@@ chgrp protein /projects/compbio/tmp/blast-prefilter-eclipse-19434 /projects/compbio/experiments/models.97/scripts2k/blast-prefilter: E-value thresholds used are For /projects/compbio/tmp/target2k-eclipse-24544/prefilter_1.fa, 1e-44 For /projects/compbio/tmp/target2k-eclipse-24544/prefilter_2.fa, 1e-40 For /projects/compbio/tmp/target2k-eclipse-24544/prefilter_3.fa, 1e-25 /projects/compbio/experiments/models.97/scripts2k/blast-prefilter: db=/projects/compbio/data/nrp/nr @@@@ uniqueseq init -alignfile /projects/compbio/tmp/target2k-eclipse-24544/m0.a2m -a protein -a2mdots 0 SAM: uniqueseq v3.2 (July 31, 2000) compiled 07/31/00_14:08:59 Reading alignment file /projects/compbio/tmp/target2k-eclipse-24544/m0.a2m (1 sequences, 230 columns) as A2M alignment. Writing sequence output to init.a2m. No sequences have been dropped. @@@@ /projects/compbio/bin/wu-blastp /projects/compbio/data/nrp/nr 1.fasta E=1e-25 V=10000 B=0 -gi > 1.fasta-blast.out Searched 551100 sequences # parsing wu-blastp output @@@@ rm -f 1.fasta < /dev/null @@@@ rm -f 1.fasta-blast.out < /dev/null # Extracting hits in FASTA format from /projects/compbio/data/nrp/nr @@@@ rm -rf /projects/compbio/tmp/blast-prefilter-eclipse-19434 < /dev/null @@@@ modelfromalign unused -alignfile m0.a2m \ -constraints_from_align 1 -constraints_out m0.cst SAM: modelfromalign v3.2 (July 31, 2000) compiled 07/31/00_14:08:27 Reading alignment file m0.a2m (1 sequences, 230 columns) as A2M alignment. # About to count sequences in m0.a2m # About to count sequences in prefilter_1.fa @@@@ modelfromalign tmp_1-a -alignfile m0.a2m \ -insert /projects/compbio/lib/gap1.5.regularizer \ -aweight_bits 0.8\ -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1 \ -aweight_method 1\ -aweight_exponent 10 -constraints m0.cst -constraints_out tmp_1-a.cst Reading parameter file /projects/compbio/lib/gap1.5.regularizer /projects/compbio/lib/gap1.5.regularizer(1): Reading REGULARIZER: gap1.5. Weak regularizer, gaps average 1.5, matches average 10 SAM: modelfromalign v3.2 (July 31, 2000) compiled 07/31/00_14:08:27 Reading alignment file m0.a2m (1 sequences, 230 columns) as A2M alignment. @@@@ hmmscore tmp_1-a -i tmp_1-a.mod -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1\ -dpstyle 0 \ -subtract_null 4 -sort 2 \ -db_size 551100 \ -simple_threshold -118.533415651609 -Emax 1e-44 \ -adpstyle 1 \ -align_short 0 -mdEmax 1e-44\ -select_score 4 \ -select_seq 4 \ -select_mdalign 4 -select_md 4 -Motifcutoff 0.0869565217391304 -db prefilter_1.fa -constraints tmp_1-a.cst -constraints_out tmp_1-a-md.cst Reading parameter file tmp_1-a.mod tmp_1-a.mod(21): Reading MODEL -- Model from alignment file m0.a2m SAM: hmmscore v3.2 (July 31, 2000) compiled 08/04/00_11:31:33 Calculating Distances (. = 1000 sequences) sh: 24543 Floating exception *** Error: /projects/compbio/experiments/models.97/scripts2k/target2k: hmmscore to select sequences failed make: *** [T0121-1-230.t2k.a2m.gz] Error 2 make -k T0121-1-230.t2k.a2m.gz \ T0121-1-230.t2k.pa \ T0121-1-230.t2k.pa.html \ T0121-1-230.t2k.2d \ T0121-1-230.t2k.tree \ T0121-1-230.t2k_sorted.pa \ T0121-1-230.t2k.tree.ps \ T0121-1-230.t2k.tree-unroot.ps make[1]: Entering directory `/export/projects/compbio/experiments/casp4/t121/t121-1-230' /projects/compbio/experiments/models.97/scripts2k/target2k -out T0121-1-230.t2k \ -seed T0121-1-230.seq \ -family \ -thresholds 1e-44,1e-40,1e-25 \ -prefilter_thresholds 1e-25,1e-20,1e-20 \ -all @@@@ chgrp protein /projects/compbio/tmp/target2k-beta-3231 @@@@ cp /export/projects/compbio/experiments/casp4/t121/t121-1-230/T0121-1-230.seq m0.a2m @@@@ checkseq foo -alphabet protein -db m0.a2m > init.check SAM: checkseq v3.2 (July 31, 2000) compiled 07/31/00_14:08:52 # About to count sequences in /projects/compbio/data/nrp/nr /projects/compbio/experiments/models.97/scripts2k/target2k: E-value thresholds used are prefilter: 1e-25, actual: 1e-44 prefilter: 1e-20, actual: 1e-40 prefilter: 1e-20, actual: 1e-25 /projects/compbio/experiments/models.97/scripts2k/target2k: aweight_bits are 0.8, 0.7, 0.5 /projects/compbio/experiments/models.97/scripts2k/target2k: db_size= 551100 reverse_diff= 4 /projects/compbio/experiments/models.97/scripts2k/target2k: blast_max_report= 10000 /projects/compbio/experiments/models.97/scripts2k/target2k: db=/projects/compbio/data/nrp/nr /projects/compbio/experiments/models.97/scripts2k/target2k: aweight_method=1 aweight_exponent=10 /projects/compbio/experiments/models.97/scripts2k/target2k: force_seed=1 constraints=1 /projects/compbio/experiments/models.97/scripts2k/target2k: jump_in=0.2 jump_out=1 fimstrength=1 fimtrans=-1 @@@@ blast-prefilter -out prefilter -seed m0.a2m -prefilter_thresholds 1e-25,1e-20,1e-20 -db /projects/compbio/data/nrp/nr -blast_max_report 10000 @@@@ chgrp protein /projects/compbio/tmp/blast-prefilter-beta-22385 /projects/compbio/experiments/models.97/scripts2k/blast-prefilter: E-value thresholds used are For /export/projects/compbio/tmp/target2k-beta-3231/prefilter_1.fa, 1e-25 For /export/projects/compbio/tmp/target2k-beta-3231/prefilter_2.fa, 1e-20 For /export/projects/compbio/tmp/target2k-beta-3231/prefilter_3.fa, 1e-20 /projects/compbio/experiments/models.97/scripts2k/blast-prefilter: db=/projects/compbio/data/nrp/nr @@@@ uniqueseq init -alignfile /export/projects/compbio/tmp/target2k-beta-3231/m0.a2m -a protein -a2mdots 0 SAM: uniqueseq v3.2 (July 31, 2000) compiled 07/31/00_14:08:59 Reading alignment file /export/projects/compbio/tmp/target2k-beta-3231/m0.a2m (1 sequences, 230 columns) as A2M alignment. Writing sequence output to init.a2m. No sequences have been dropped. @@@@ /projects/compbio/bin/wu-blastp /projects/compbio/data/nrp/nr 1.fasta E=1e-20 V=10000 B=0 -gi > 1.fasta-blast.out Searched 551100 sequences # parsing wu-blastp output @@@@ rm -f 1.fasta < /dev/null @@@@ rm -f 1.fasta-blast.out < /dev/null # Extracting hits in FASTA format from /projects/compbio/data/nrp/nr @@@@ rm -rf /projects/compbio/tmp/blast-prefilter-beta-22385 < /dev/null @@@@ modelfromalign unused -alignfile m0.a2m \ -constraints_from_align 1 -constraints_out m0.cst SAM: modelfromalign v3.2 (July 31, 2000) compiled 07/31/00_14:08:27 Reading alignment file m0.a2m (1 sequences, 230 columns) as A2M alignment. # About to count sequences in m0.a2m # About to count sequences in prefilter_1.fa @@@@ modelfromalign tmp_1-a -alignfile m0.a2m \ -insert /projects/compbio/lib/gap1.5.regularizer \ -aweight_bits 0.8\ -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1 \ -aweight_method 1\ -aweight_exponent 10 -constraints m0.cst -constraints_out tmp_1-a.cst Reading parameter file /projects/compbio/lib/gap1.5.regularizer /projects/compbio/lib/gap1.5.regularizer(1): Reading REGULARIZER: gap1.5. Weak regularizer, gaps average 1.5, matches average 10 SAM: modelfromalign v3.2 (July 31, 2000) compiled 07/31/00_14:08:27 Reading alignment file m0.a2m (1 sequences, 230 columns) as A2M alignment. @@@@ hmmscore tmp_1-a -i tmp_1-a.mod -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1\ -dpstyle 0 \ -subtract_null 4 -sort 2 \ -db_size 551100 \ -simple_threshold -118.533415651609 -Emax 1e-44 \ -adpstyle 1 \ -align_short 0 -mdEmax 1e-44\ -select_score 4 \ -select_seq 4 \ -select_mdalign 4 -select_md 4 -Motifcutoff 0.0869565217391304 -db prefilter_1.fa -constraints tmp_1-a.cst -constraints_out tmp_1-a-md.cst Reading parameter file tmp_1-a.mod tmp_1-a.mod(21): Reading MODEL -- Model from alignment file m0.a2m SAM: hmmscore v3.2 (July 31, 2000) compiled 08/04/00_11:31:33 Calculating Distances (. = 1000 sequences) sh: 1982 Floating exception *** Error: /projects/compbio/experiments/models.97/scripts2k/target2k: hmmscore to select sequences failed make[1]: *** [T0121-1-230.t2k.a2m.gz] Error 2 make[1]: Target `T0121-1-230.t2k.pa' not remade because of errors. make[1]: Target `T0121-1-230.t2k.pa.html' not remade because of errors. make[1]: Target `T0121-1-230.t2k.2d' not remade because of errors. make[1]: Target `T0121-1-230.t2k.tree' not remade because of errors. make[1]: Target `T0121-1-230.t2k_sorted.pa' not remade because of errors. make[1]: Target `T0121-1-230.t2k.tree.ps' not remade because of errors. make[1]: Target `T0121-1-230.t2k.tree-unroot.ps' not remade because of errors. make[1]: Leaving directory `/export/projects/compbio/experiments/casp4/t121/t121-1-230' make: [T0121-1-230.align] Error 2 (ignored) /projects/compbio/experiments/models.97/scripts2k/target2k -out T0121-1-230.t2k \ -seed T0121-1-230.seq \ -family \ -thresholds 1e-44,1e-40,1e-25 \ -prefilter_thresholds 1e-25,1e-20,1e-20 \ -all @@@@ chgrp protein /projects/compbio/tmp/target2k-beta-1520 @@@@ cp /export/projects/compbio/experiments/casp4/t121/t121-1-230/T0121-1-230.seq m0.a2m @@@@ checkseq foo -alphabet protein -db m0.a2m > init.check SAM: checkseq v3.2 (July 31, 2000) compiled 07/31/00_14:08:52 # About to count sequences in /projects/compbio/data/nrp/nr /projects/compbio/experiments/models.97/scripts2k/target2k: E-value thresholds used are prefilter: 1e-25, actual: 1e-44 prefilter: 1e-20, actual: 1e-40 prefilter: 1e-20, actual: 1e-25 /projects/compbio/experiments/models.97/scripts2k/target2k: aweight_bits are 0.8, 0.7, 0.5 /projects/compbio/experiments/models.97/scripts2k/target2k: db_size= 551100 reverse_diff= 4 /projects/compbio/experiments/models.97/scripts2k/target2k: blast_max_report= 10000 /projects/compbio/experiments/models.97/scripts2k/target2k: db=/projects/compbio/data/nrp/nr /projects/compbio/experiments/models.97/scripts2k/target2k: aweight_method=1 aweight_exponent=10 /projects/compbio/experiments/models.97/scripts2k/target2k: force_seed=1 constraints=1 /projects/compbio/experiments/models.97/scripts2k/target2k: jump_in=0.2 jump_out=1 fimstrength=1 fimtrans=-1 @@@@ blast-prefilter -out prefilter -seed m0.a2m -prefilter_thresholds 1e-25,1e-20,1e-20 -db /projects/compbio/data/nrp/nr -blast_max_report 10000 @@@@ chgrp protein /projects/compbio/tmp/blast-prefilter-beta-31482 /projects/compbio/experiments/models.97/scripts2k/blast-prefilter: E-value thresholds used are For /export/projects/compbio/tmp/target2k-beta-1520/prefilter_1.fa, 1e-25 For /export/projects/compbio/tmp/target2k-beta-1520/prefilter_2.fa, 1e-20 For /export/projects/compbio/tmp/target2k-beta-1520/prefilter_3.fa, 1e-20 /projects/compbio/experiments/models.97/scripts2k/blast-prefilter: db=/projects/compbio/data/nrp/nr @@@@ uniqueseq init -alignfile /export/projects/compbio/tmp/target2k-beta-1520/m0.a2m -a protein -a2mdots 0 SAM: uniqueseq v3.2 (July 31, 2000) compiled 07/31/00_14:08:59 Reading alignment file /export/projects/compbio/tmp/target2k-beta-1520/m0.a2m (1 sequences, 230 columns) as A2M alignment. Writing sequence output to init.a2m. No sequences have been dropped. @@@@ /projects/compbio/bin/wu-blastp /projects/compbio/data/nrp/nr 1.fasta E=1e-20 V=10000 B=0 -gi > 1.fasta-blast.out Searched 551100 sequences # parsing wu-blastp output @@@@ rm -f 1.fasta < /dev/null @@@@ rm -f 1.fasta-blast.out < /dev/null # Extracting hits in FASTA format from /projects/compbio/data/nrp/nr @@@@ rm -rf /projects/compbio/tmp/blast-prefilter-beta-31482 < /dev/null @@@@ modelfromalign unused -alignfile m0.a2m \ -constraints_from_align 1 -constraints_out m0.cst SAM: modelfromalign v3.2 (July 31, 2000) compiled 07/31/00_14:08:27 Reading alignment file m0.a2m (1 sequences, 230 columns) as A2M alignment. # About to count sequences in m0.a2m # About to count sequences in prefilter_1.fa @@@@ modelfromalign tmp_1-a -alignfile m0.a2m \ -insert /projects/compbio/lib/gap1.5.regularizer \ -aweight_bits 0.8\ -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1 \ -aweight_method 1\ -aweight_exponent 10 -constraints m0.cst -constraints_out tmp_1-a.cst Reading parameter file /projects/compbio/lib/gap1.5.regularizer /projects/compbio/lib/gap1.5.regularizer(1): Reading REGULARIZER: gap1.5. Weak regularizer, gaps average 1.5, matches average 10 SAM: modelfromalign v3.2 (July 31, 2000) compiled 07/31/00_14:08:27 Reading alignment file m0.a2m (1 sequences, 230 columns) as A2M alignment. @@@@ hmmscore tmp_1-a -i tmp_1-a.mod -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1\ -dpstyle 0 \ -subtract_null 4 -sort 2 \ -db_size 551100 \ -simple_threshold -118.533415651609 -Emax 1e-44 \ -adpstyle 1 \ -align_short 0 -mdEmax 1e-44\ -select_score 4 \ -select_seq 4 \ -select_mdalign 4 -select_md 4 -Motifcutoff 0.0869565217391304 -db prefilter_1.fa -constraints tmp_1-a.cst -constraints_out tmp_1-a-md.cst Reading parameter file tmp_1-a.mod tmp_1-a.mod(21): Reading MODEL -- Model from alignment file m0.a2m SAM: hmmscore v3.2 (July 31, 2000) compiled 08/04/00_11:31:33 Calculating Distances (. = 1000 sequences) sh: 1088 Floating exception *** Error: /projects/compbio/experiments/models.97/scripts2k/target2k: hmmscore to select sequences failed make: *** [T0121-1-230.t2k.a2m.gz] Error 2