make -k T0115-outer-blast.rdb T0115-outer-double-blast.rdb make[1]: Entering directory `/auto/projects/compbio/experiments/casp4/t115/t115-outer' /projects/compbio/experiments/casp4/scripts/single-blast -q T0115-outer.seq -interdb /projects/compbio/data/nrp/nr -db /projects/compbio/data/pdb/all-protein > T0115-outer-blast.rdb starting T0115-outer with E=10 WARNING: One or more instances of an invalid amino acid code "O" have been encountered in the query sequence and are being skipped. /projects/compbio/experiments/casp4/scripts/double-blast -q T0115-outer.seq -interdb /projects/compbio/data/nrp/nr -db /projects/compbio/data/pdb/all-protein > T0115-outer-double-blast.rdb starting T0115-outer with E=0.000050 WARNING: One or more instances of an invalid amino acid code "O" have been encountered in the query sequence and are being skipped. T0115-outer T0115-outer blasting: WARNING: One or more instances of an invalid amino acid code "O" have been encountered in the query sequence and are being skipped. T0115-outer gi|4614|emb|CAA37083.1|_6:156 blasting: T0115-outer gi|4614|emb|CAA37083.1|_303:338 blasting: T0115-outer gi|125390|sp|P07128|KHSE_BRELA_10:149 blasting: T0115-outer gi|125390|sp|P07128|KHSE_BRELA_259:304 blasting: T0115-outer gi|125391|sp|P08210|KHSE_CORGL_10:149 blasting: T0115-outer gi|125391|sp|P08210|KHSE_CORGL_259:304 blasting: T0115-outer gi|125393|sp|P04947|KHSE_FREDI_1:145 blasting: T0115-outer gi|125393|sp|P04947|KHSE_FREDI_251:264 blasting: T0115-outer gi|125395|sp|P27722|KHSE_SERMA_2:130 blasting: T0115-outer gi|125395|sp|P27722|KHSE_SERMA_259:268 blasting: T0115-outer gi|147980|gb|AAA83915.1|_2:130 blasting: T0115-outer gi|147980|gb|AAA83915.1|_258:289 blasting: T0115-outer gi|266419|sp|P00547|KHSE_ECOLI_2:130 blasting: T0115-outer gi|266419|sp|P00547|KHSE_ECOLI_259:290 blasting: T0115-outer gi|586879|sp|P37550|IPK_BACSU_39:150 blasting: T0115-outer gi|586879|sp|P37550|IPK_BACSU_243:272 blasting: T0115-outer gi|1170651|sp|P45836|KHSE_MYCLE_18:137 blasting: T0115-outer gi|1170651|sp|P45836|KHSE_MYCLE_249:273 blasting: T0115-outer gi|1170650|sp|P44504|KHSE_HAEIN_2:187 blasting: T0115-outer gi|1730045|sp|P52991|KHSE_LACLA_1:156 blasting: T0115-outer gi|1730045|sp|P52991|KHSE_LACLA_243:271 blasting: T0115-outer gi|1730046|sp|Q10603|KHSE_MYCTU_20:139 blasting: T0115-outer gi|1730046|sp|Q10603|KHSE_MYCTU_251:288 blasting: T0115-outer gi|2497513|sp|P72535|KHSE_STRPN_1:161 blasting: T0115-outer gi|2497516|sp|Q92209|KHSE_CANAL_3:145 blasting: T0115-outer gi|2497515|sp|Q58504|KHSE_METJA_1:133 blasting: T0115-outer gi|2497515|sp|Q58504|KHSE_METJA_157:300 blasting: T0115-outer gi|2660644|emb|CAA04516.1|_68:127 blasting: T0115-outer gi|7490646|pir||T40495_1:174 blasting: T0115-outer gi|6225592|sp|O67332|KHSE_AQUAE_2:185 blasting: T0115-outer gi|7450512|pir||D71103_1:100 blasting: T0115-outer gi|7450512|pir||D71103_222:259 blasting: T0115-outer gi|4927412|gb|AAD33097.1|AF082525_1_52:188 blasting: T0115-outer gi|4927412|gb|AAD33097.1|AF082525_1_310:323 blasting: T0115-outer gi|7450508|pir||F72364_1:155 blasting: T0115-outer gi|7521143|pir||E72511_35:165 blasting: T0115-outer gi|7521143|pir||E72511_291:342 blasting: T0115-outer gi|7434441|pir||H75080_1:125 blasting: T0115-outer gi|7434441|pir||H75080_244:281 blasting: T0115-outer gi|7472024|pir||G75280_10:137 blasting: T0115-outer gi|7472024|pir||G75280_251:264 blasting: T0115-outer gi|7532410|gb|AAF63224.1|_52:188 blasting: T0115-outer gi|7532410|gb|AAF63224.1|_310:323 blasting: T0115-outer gi|9107373|gb|AAF85023.1|AE004035_2_20:156 blasting: T0115-outer gi|9107373|gb|AAF85023.1|AE004035_2_258:282 blasting: T0115-outer gi|9656932|gb|AAF95506.1|_1:142 blasting: T0115-outer gi|9656932|gb|AAF95506.1|_265:299 blasting: make[1]: Leaving directory `/auto/projects/compbio/experiments/casp4/t115/t115-outer' make -k T0115-outer.t2k.a2m.gz \ T0115-outer.t2k.pa \ T0115-outer.t2k.pa.html \ T0115-outer.t2k.2d \ T0115-outer.t2k.tree \ T0115-outer.t2k_sorted.pa \ T0115-outer.t2k.tree.ps \ T0115-outer.t2k.tree-unroot.ps make[1]: Entering directory `/auto/projects/compbio/experiments/casp4/t115/t115-outer' /projects/compbio/experiments/models.97/scripts2k/target2k -out T0115-outer.t2k \ -seed T0115-outer.seq \ -all @@@@ chgrp protein /projects/compbio/tmp/target2k-eclipse-21584 @@@@ cp /projects/compbio/experiments/casp4/t115/t115-outer/T0115-outer.seq m0.a2m @@@@ checkseq foo -alphabet protein -db m0.a2m > init.check SAM: checkseq v3.2 (July 31, 2000) compiled 07/31/00_14:08:52 # About to count sequences in /projects/compbio/data/nrp/nr /projects/compbio/experiments/models.97/scripts2k/target2k: E-value thresholds used are prefilter: 0.01, actual: 0.0001 prefilter: 1, actual: 0.0002 prefilter: 10, actual: 0.001 prefilter: 400, actual: 0.005 /projects/compbio/experiments/models.97/scripts2k/target2k: aweight_bits are 0.8, 0.7, 0.6, 0.5 /projects/compbio/experiments/models.97/scripts2k/target2k: db_size= 551100 reverse_diff= 4 /projects/compbio/experiments/models.97/scripts2k/target2k: blast_max_report= 20000 /projects/compbio/experiments/models.97/scripts2k/target2k: db=/projects/compbio/data/nrp/nr /projects/compbio/experiments/models.97/scripts2k/target2k: aweight_method=1 aweight_exponent=10 /projects/compbio/experiments/models.97/scripts2k/target2k: force_seed=1 constraints=1 /projects/compbio/experiments/models.97/scripts2k/target2k: jump_in=0.2 jump_out=1 fimstrength=1 fimtrans=-1 @@@@ blast-prefilter -out prefilter -seed m0.a2m -prefilter_thresholds 0.01,1,10,400 -db /projects/compbio/data/nrp/nr -blast_max_report 20000 @@@@ chgrp protein /projects/compbio/tmp/blast-prefilter-eclipse-5891 /projects/compbio/experiments/models.97/scripts2k/blast-prefilter: E-value thresholds used are For /projects/compbio/tmp/target2k-eclipse-21584/prefilter_1.fa, 0.01 For /projects/compbio/tmp/target2k-eclipse-21584/prefilter_2.fa, 1 For /projects/compbio/tmp/target2k-eclipse-21584/prefilter_3.fa, 10 For /projects/compbio/tmp/target2k-eclipse-21584/prefilter_4.fa, 400 /projects/compbio/experiments/models.97/scripts2k/blast-prefilter: db=/projects/compbio/data/nrp/nr @@@@ uniqueseq init -alignfile /projects/compbio/tmp/target2k-eclipse-21584/m0.a2m -a protein -a2mdots 0 SAM: uniqueseq v3.2 (July 31, 2000) compiled 07/31/00_14:08:59 Reading alignment file /projects/compbio/tmp/target2k-eclipse-21584/m0.a2m (1 sequences, 184 columns) as A2M alignment. Writing sequence output to init.a2m. No sequences have been dropped. @@@@ /projects/compbio/bin/wu-blastp /projects/compbio/data/nrp/nr 1.fasta E=400 V=20000 B=0 -gi > 1.fasta-blast.out Searched 551100 sequences # parsing wu-blastp output @@@@ rm -f 1.fasta < /dev/null @@@@ rm -f 1.fasta-blast.out < /dev/null # Extracting hits in FASTA format from /projects/compbio/data/nrp/nr @@@@ rm -rf /projects/compbio/tmp/blast-prefilter-eclipse-5891 < /dev/null @@@@ modelfromalign unused -alignfile m0.a2m \ -constraints_from_align 1 -constraints_out m0.cst SAM: modelfromalign v3.2 (July 31, 2000) compiled 07/31/00_14:08:27 Reading alignment file m0.a2m (1 sequences, 184 columns) as A2M alignment. Node 134 is a FIM. # About to count sequences in m0.a2m # About to count sequences in prefilter_1.fa @@@@ modelfromalign tmp_1-a -alignfile m0.a2m \ -insert /projects/compbio/lib/gap1.5.regularizer \ -aweight_bits 0.8\ -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1 \ -aweight_method 1\ -aweight_exponent 10 -constraints m0.cst -constraints_out tmp_1-a.cst Reading parameter file /projects/compbio/lib/gap1.5.regularizer /projects/compbio/lib/gap1.5.regularizer(1): Reading REGULARIZER: gap1.5. Weak regularizer, gaps average 1.5, matches average 10 SAM: modelfromalign v3.2 (July 31, 2000) compiled 07/31/00_14:08:27 Reading alignment file m0.a2m (1 sequences, 184 columns) as A2M alignment. Node 134 is a FIM. @@@@ hmmscore tmp_1-a -i tmp_1-a.mod -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1\ -dpstyle 0 \ -subtract_null 4 -sort 2 \ -db_size 551100 \ -simple_threshold -26.4300119316661 -Emax 0.0001 \ -adpstyle 1 \ -align_short 0 -mdEmax 0.0001\ -select_score 4 \ -select_seq 4 \ -select_mdalign 4 -select_md 4 -Motifcutoff 0.108695652173913 -db prefilter_1.fa -constraints tmp_1-a.cst -constraints_out tmp_1-a-md.cst Reading parameter file tmp_1-a.mod tmp_1-a.mod(21): Reading MODEL -- Model from alignment file m0.a2m SAM: hmmscore v3.2 (July 31, 2000) compiled 08/04/00_11:31:33 Some FIMs have non-zero match states (e.g., node 134), a natural result of turning an existing or GENERIC node into a FIM. These nodes are being renormalized as discussed in the SAM manual. To avoid this message, use addfims or hmmedit when adding FIMs. Calculating Distances (. = 1000 sequences) . Average NLL-Simple NULL score: -59.506287 Database has 33 sequences with 10057 residues. @@@@ sortseq tmp_1-a-sort -alignfile tmp_1-a.mult \ -NLLfile tmp_1-a.mstat -a2mdots 0 SAM: sortseq v3.2 (July 31, 2000) compiled 07/31/00_14:08:48 Reading alignment file tmp_1-a.mult (31 sequences, 184 columns) as A2M alignment. Reading scores from file tmp_1-a.mstat Writing sequence output to tmp_1-a-sort.a2m. @@@@ cat m0.a2m tmp_1-a-sort.a2m > tmp_1-a.train.seq @@@@ cat m0.cst tmp_1-a-md.cst > tmp_1-a.train.cst # About to count sequences in tmp_1-a.train.seq # num_seqs=32 nll_thresh=-22.4300119316661 frac_id=0.999277956550716 @@@@ uniqueseq thinned_tmp -alignfile tmp_1-a.train.seq -a protein \ -percent_id 0.999277956550716 -a2mdots 0 SAM: uniqueseq v3.2 (July 31, 2000) compiled 07/31/00_14:08:59 Reading alignment file tmp_1-a.train.seq (32 sequences, 184 columns) as A2M alignment. Writing sequence output to thinned_tmp.a2m. Dropping 1 (of 32) duplicate sequences with differing IDs Dropping 3 (of 32) sequences with > 99.9% id 28 sequences left after dropping 4 of 32 sequences. @@@@ modelfromalign junk_for_weights \ -alignfile tmp_1-a.train.seq \ -aweight_bits 0.8\ -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1 \ -aweight_method 1\ -aweight_exponent 10 SAM: modelfromalign v3.2 (July 31, 2000) compiled 07/31/00_14:08:27 Reading alignment file tmp_1-a.train.seq (28 sequences, 184 columns) as A2M alignment. @@@@ buildmodel tmp_1-b -insert tmp_1-a.mod\ -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1\ -modellength 0 \ -internal_weight 0 \ -anneal_length 2 \ -many_files 1 \ -Nseq 20000\ -nsurgery 0 \ -fracinsert 0.2 \ -cutinsert 0.25 -cutmatch 0.2 -initial_noise 0.0001 -anneal_noise 0.000001 \ -internal_weight 0 \ -train tmp_1-a.train.seq \ -nmodels 1\ -insert /projects/compbio/lib/gap1.5.regularizer -sequence_weights tmp_1-a.w -constraints tmp_1-a.train.cst Reading parameter file tmp_1-a.mod tmp_1-a.mod(21): Reading MODEL -- Model from alignment file m0.a2m Command line option(48): internal_weight has already been set. Using 0. Reading parameter file /projects/compbio/lib/gap1.5.regularizer /projects/compbio/lib/gap1.5.regularizer(1): Reading REGULARIZER: gap1.5. Weak regularizer, gaps average 1.5, matches average 10 SAM: buildmodel v3.2 (July 31, 2000) compiled 07/31/00_14:08:12 Some FIMs have non-zero match states (e.g., node 134), a natural result of turning an existing or GENERIC node into a FIM. These nodes are being renormalized as discussed in the SAM manual. To avoid this message, use addfims or hmmedit when adding FIMs. -437.71 -40.05 -144.97 67.49 13 0 185 @@@@ hmmscore tmp_1-b -i tmp_1-b.mod -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1\ -dpstyle 0 \ -subtract_null 4 -sort 2 \ -db_size 551100 \ -db m0.a2m -db tmp_1-a.sel \ -select_score 4 \ -select_seq 4 \ -simple_threshold -26.4300119316661 -Emax 0.0001 \ -select_mdalign 4 -select_md 4 \ -adpstyle 1 \ -Motifcutoff 0.108695652173913 \ -align_short 5 -mdEmax 0.0001 -constraints tmp_1-a.train.cst -constraints_out /projects/compbio/experiments/casp4/t115/t115-outer/T0115-outer.t2k_1.cst Reading parameter file tmp_1-b.mod tmp_1-b.mod(18): Reading MODEL -- Final model for run tmp_1-b SAM: hmmscore v3.2 (July 31, 2000) compiled 08/04/00_11:31:33 Calculating Distances (. = 1000 sequences) . Average NLL-Simple NULL score: -156.289551 Database has 32 sequences with 9841 residues. @@@@ cp -f tmp_1-b.mult /projects/compbio/experiments/casp4/t115/t115-outer/T0115-outer.t2k_1.a2m # About to count sequences in prefilter_2.fa @@@@ modelfromalign tmp_2-a -alignfile /projects/compbio/experiments/casp4/t115/t115-outer/T0115-outer.t2k_1.a2m \ -insert /projects/compbio/lib/stiff-gap5.regularizer \ -aweight_bits 0.7\ -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1 \ -aweight_method 1\ -aweight_exponent 10 -constraints /projects/compbio/experiments/casp4/t115/t115-outer/T0115-outer.t2k_1.cst -constraints_out tmp_2-a.cst Reading parameter file /projects/compbio/lib/stiff-gap5.regularizer /projects/compbio/lib/stiff-gap5.regularizer(1): Reading REGULARIZER: gap5. stiff regularizer, gaps averge 5, matches average 10 SAM: modelfromalign v3.2 (July 31, 2000) compiled 07/31/00_14:08:27 Reading alignment file /projects/compbio/experiments/casp4/t115/t115-outer/T0115-outer.t2k_1.a2m (34 sequences, 184 columns) as A2M alignment. @@@@ hmmscore tmp_2-a -i tmp_2-a.mod -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1\ -dpstyle 0 \ -subtract_null 4 -sort 2 \ -db_size 551100 \ -simple_threshold -25.7368647509247 -Emax 0.0002 \ -adpstyle 1 \ -align_short 0 -mdEmax 0.0002\ -select_score 4 \ -select_seq 4 \ -select_mdalign 4 -select_md 4 -Motifcutoff 0.108695652173913 -db prefilter_2.fa -constraints tmp_2-a.cst -constraints_out tmp_2-a-md.cst Reading parameter file tmp_2-a.mod tmp_2-a.mod(21): Reading MODEL -- Model from alignment file /projects/compbio/experiments/casp4/t115/t115-outer/T0115-outer.t2k_1.a2m SAM: hmmscore v3.2 (July 31, 2000) compiled 08/04/00_11:31:33 Calculating Distances (. = 1000 sequences) . Average NLL-Simple NULL score: -92.883316 Database has 64 sequences with 19567 residues. @@@@ sortseq tmp_2-a-sort -alignfile tmp_2-a.mult \ -NLLfile tmp_2-a.mstat -a2mdots 0 SAM: sortseq v3.2 (July 31, 2000) compiled 07/31/00_14:08:48 Reading alignment file tmp_2-a.mult (56 sequences, 184 columns) as A2M alignment. Reading scores from file tmp_2-a.mstat Writing sequence output to tmp_2-a-sort.a2m. @@@@ cat m0.a2m tmp_2-a-sort.a2m > tmp_2-a.train.seq @@@@ cat m0.cst tmp_2-a-md.cst > tmp_2-a.train.cst # About to count sequences in tmp_2-a.train.seq # num_seqs=57 nll_thresh=-21.7368647509247 frac_id=0.982724391394556 @@@@ uniqueseq thinned_tmp -alignfile tmp_2-a.train.seq -a protein \ -percent_id 0.982724391394556 -a2mdots 0 SAM: uniqueseq v3.2 (July 31, 2000) compiled 07/31/00_14:08:59 Reading alignment file tmp_2-a.train.seq (57 sequences, 184 columns) as A2M alignment. Writing sequence output to thinned_tmp.a2m. Dropping 5 (of 57) sequences with > 98.3% id 52 sequences left after dropping 5 of 57 sequences. @@@@ modelfromalign junk_for_weights \ -alignfile tmp_2-a.train.seq \ -aweight_bits 0.7\ -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1 \ -aweight_method 1\ -aweight_exponent 10 SAM: modelfromalign v3.2 (July 31, 2000) compiled 07/31/00_14:08:27 Reading alignment file tmp_2-a.train.seq (52 sequences, 184 columns) as A2M alignment. @@@@ buildmodel tmp_2-b -insert tmp_2-a.mod\ -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1\ -modellength 0 \ -internal_weight 0 \ -anneal_length 2 \ -many_files 1 \ -Nseq 20000\ -nsurgery 0 \ -fracinsert 0.2 \ -cutinsert 0.25 -cutmatch 0.2 -initial_noise 0.0001 -anneal_noise 0.000001 \ -internal_weight 0 \ -train tmp_2-a.train.seq \ -nmodels 1\ -insert /projects/compbio/lib/stiff-gap5.regularizer -sequence_weights tmp_2-a.w -constraints tmp_2-a.train.cst Reading parameter file tmp_2-a.mod tmp_2-a.mod(21): Reading MODEL -- Model from alignment file /projects/compbio/experiments/casp4/t115/t115-outer/T0115-outer.t2k_1.a2m Command line option(48): internal_weight has already been set. Using 0. Reading parameter file /projects/compbio/lib/stiff-gap5.regularizer /projects/compbio/lib/stiff-gap5.regularizer(1): Reading REGULARIZER: gap5. stiff regularizer, gaps averge 5, matches average 10 SAM: buildmodel v3.2 (July 31, 2000) compiled 07/31/00_14:08:12 -436.51 -18.98 -103.34 59.12 6 0 185 @@@@ hmmscore tmp_2-b -i tmp_2-b.mod -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1\ -dpstyle 0 \ -subtract_null 4 -sort 2 \ -db_size 551100 \ -db m0.a2m -db tmp_2-a.sel \ -select_score 4 \ -select_seq 4 \ -simple_threshold -25.7368647509247 -Emax 0.0002 \ -select_mdalign 4 -select_md 4 \ -adpstyle 1 \ -Motifcutoff 0.108695652173913 \ -align_short 5 -mdEmax 0.0002 -constraints tmp_2-a.train.cst -constraints_out /projects/compbio/experiments/casp4/t115/t115-outer/T0115-outer.t2k_2.cst Reading parameter file tmp_2-b.mod tmp_2-b.mod(18): Reading MODEL -- Final model for run tmp_2-b SAM: hmmscore v3.2 (July 31, 2000) compiled 08/04/00_11:31:33 Calculating Distances (. = 1000 sequences) . Average NLL-Simple NULL score: -112.331696 Database has 58 sequences with 17782 residues. @@@@ cp -f tmp_2-b.mult /projects/compbio/experiments/casp4/t115/t115-outer/T0115-outer.t2k_2.a2m # About to count sequences in prefilter_3.fa @@@@ modelfromalign tmp_3-a -alignfile /projects/compbio/experiments/casp4/t115/t115-outer/T0115-outer.t2k_2.a2m \ -insert /projects/compbio/lib/stiff-gap5.regularizer \ -aweight_bits 0.6\ -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1 \ -aweight_method 1\ -aweight_exponent 10 -constraints /projects/compbio/experiments/casp4/t115/t115-outer/T0115-outer.t2k_2.cst -constraints_out tmp_3-a.cst Reading parameter file /projects/compbio/lib/stiff-gap5.regularizer /projects/compbio/lib/stiff-gap5.regularizer(1): Reading REGULARIZER: gap5. stiff regularizer, gaps averge 5, matches average 10 SAM: modelfromalign v3.2 (July 31, 2000) compiled 07/31/00_14:08:27 Reading alignment file /projects/compbio/experiments/casp4/t115/t115-outer/T0115-outer.t2k_2.a2m (59 sequences, 184 columns) as A2M alignment. @@@@ hmmscore tmp_3-a -i tmp_3-a.mod -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1\ -dpstyle 0 \ -subtract_null 4 -sort 2 \ -db_size 551100 \ -simple_threshold -24.1274268370389 -Emax 0.001 \ -adpstyle 1 \ -align_short 0 -mdEmax 0.001\ -select_score 4 \ -select_seq 4 \ -select_mdalign 4 -select_md 4 -Motifcutoff 0.108695652173913 -db prefilter_3.fa -constraints tmp_3-a.cst -constraints_out tmp_3-a-md.cst Reading parameter file tmp_3-a.mod tmp_3-a.mod(21): Reading MODEL -- Model from alignment file /projects/compbio/experiments/casp4/t115/t115-outer/T0115-outer.t2k_2.a2m SAM: hmmscore v3.2 (July 31, 2000) compiled 08/04/00_11:31:33 Calculating Distances (. = 1000 sequences) . Average NLL-Simple NULL score: -72.654350 Database has 104 sequences with 31272 residues. @@@@ sortseq tmp_3-a-sort -alignfile tmp_3-a.mult \ -NLLfile tmp_3-a.mstat -a2mdots 0 SAM: sortseq v3.2 (July 31, 2000) compiled 07/31/00_14:08:48 Reading alignment file tmp_3-a.mult (77 sequences, 184 columns) as A2M alignment. Reading scores from file tmp_3-a.mstat Writing sequence output to tmp_3-a-sort.a2m. @@@@ cat m0.a2m tmp_3-a-sort.a2m > tmp_3-a.train.seq @@@@ cat m0.cst tmp_3-a-md.cst > tmp_3-a.train.cst # About to count sequences in tmp_3-a.train.seq # num_seqs=78 nll_thresh=-20.1274268370389 frac_id=0.943673623611722 @@@@ uniqueseq thinned_tmp -alignfile tmp_3-a.train.seq -a protein \ -percent_id 0.943673623611722 -a2mdots 0 SAM: uniqueseq v3.2 (July 31, 2000) compiled 07/31/00_14:08:59 Reading alignment file tmp_3-a.train.seq (78 sequences, 184 columns) as A2M alignment. Writing sequence output to thinned_tmp.a2m. Dropping 4 (of 78) duplicate sequences with differing IDs Dropping 12 (of 78) sequences with > 94.4% id 62 sequences left after dropping 16 of 78 sequences. @@@@ modelfromalign junk_for_weights \ -alignfile tmp_3-a.train.seq \ -aweight_bits 0.6\ -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1 \ -aweight_method 1\ -aweight_exponent 10 SAM: modelfromalign v3.2 (July 31, 2000) compiled 07/31/00_14:08:27 Reading alignment file tmp_3-a.train.seq (62 sequences, 184 columns) as A2M alignment. @@@@ buildmodel tmp_3-b -insert tmp_3-a.mod\ -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1\ -modellength 0 \ -internal_weight 0 \ -anneal_length 2 \ -many_files 1 \ -Nseq 20000\ -nsurgery 0 \ -fracinsert 0.2 \ -cutinsert 0.25 -cutmatch 0.2 -initial_noise 0.0001 -anneal_noise 0.000001 \ -internal_weight 0 \ -train tmp_3-a.train.seq \ -nmodels 1\ -insert /projects/compbio/lib/stiff-gap5.regularizer -sequence_weights tmp_3-a.w -constraints tmp_3-a.train.cst Reading parameter file tmp_3-a.mod tmp_3-a.mod(21): Reading MODEL -- Model from alignment file /projects/compbio/experiments/casp4/t115/t115-outer/T0115-outer.t2k_2.a2m Command line option(48): internal_weight has already been set. Using 0. Reading parameter file /projects/compbio/lib/stiff-gap5.regularizer /projects/compbio/lib/stiff-gap5.regularizer(1): Reading REGULARIZER: gap5. stiff regularizer, gaps averge 5, matches average 10 SAM: buildmodel v3.2 (July 31, 2000) compiled 07/31/00_14:08:12 -515.72 -13.98 -86.78 61.10 7 0 185 @@@@ hmmscore tmp_3-b -i tmp_3-b.mod -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1\ -dpstyle 0 \ -subtract_null 4 -sort 2 \ -db_size 551100 \ -db m0.a2m -db tmp_3-a.sel \ -select_score 4 \ -select_seq 4 \ -simple_threshold -24.1274268370389 -Emax 0.001 \ -select_mdalign 4 -select_md 4 \ -adpstyle 1 \ -Motifcutoff 0.108695652173913 \ -align_short 5 -mdEmax 0.001 -constraints tmp_3-a.train.cst -constraints_out /projects/compbio/experiments/casp4/t115/t115-outer/T0115-outer.t2k_3.cst Reading parameter file tmp_3-b.mod tmp_3-b.mod(18): Reading MODEL -- Final model for run tmp_3-b SAM: hmmscore v3.2 (July 31, 2000) compiled 08/04/00_11:31:33 Calculating Distances (. = 1000 sequences) . Average NLL-Simple NULL score: -97.794800 Database has 77 sequences with 23359 residues. @@@@ cp -f tmp_3-b.mult /projects/compbio/experiments/casp4/t115/t115-outer/T0115-outer.t2k_3.a2m # About to count sequences in prefilter_4.fa @@@@ modelfromalign tmp_4-a -alignfile /projects/compbio/experiments/casp4/t115/t115-outer/T0115-outer.t2k_3.a2m \ -insert /projects/compbio/lib/fssp-trained.regularizer \ -aweight_bits 0.5\ -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1 \ -aweight_method 1\ -aweight_exponent 10 -constraints /projects/compbio/experiments/casp4/t115/t115-outer/T0115-outer.t2k_3.cst -constraints_out tmp_4-a.cst Reading parameter file /projects/compbio/lib/fssp-trained.regularizer /projects/compbio/lib/fssp-trained.regularizer(1): Reading REGULARIZER: transitions from FSSP and pseudocounts for diverse weighted HSSP SAM: modelfromalign v3.2 (July 31, 2000) compiled 07/31/00_14:08:27 Reading alignment file /projects/compbio/experiments/casp4/t115/t115-outer/T0115-outer.t2k_3.a2m (77 sequences, 184 columns) as A2M alignment. @@@@ hmmscore tmp_4-a -i tmp_4-a.mod -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1\ -dpstyle 0 \ -subtract_null 4 -sort 2 \ -db_size 551100 \ -simple_threshold -22.5179889173466 -Emax 0.005 \ -adpstyle 1 \ -align_short 0 -mdEmax 0.005\ -select_score 4 \ -select_seq 4 \ -select_mdalign 4 -select_md 4 -Motifcutoff 0.108695652173913 -db prefilter_4.fa -constraints tmp_4-a.cst -constraints_out tmp_4-a-md.cst Reading parameter file tmp_4-a.mod tmp_4-a.mod(21): Reading MODEL -- Model from alignment file /projects/compbio/experiments/casp4/t115/t115-outer/T0115-outer.t2k_3.a2m SAM: hmmscore v3.2 (July 31, 2000) compiled 08/04/00_11:31:33 Calculating Distances (. = 1000 sequences) . Average NLL-Simple NULL score: -25.361258 Database has 520 sequences with 142215 residues. @@@@ sortseq tmp_4-a-sort -alignfile tmp_4-a.mult \ -NLLfile tmp_4-a.mstat -a2mdots 0 SAM: sortseq v3.2 (July 31, 2000) compiled 07/31/00_14:08:48 Reading alignment file tmp_4-a.mult (121 sequences, 184 columns) as A2M alignment. Reading scores from file tmp_4-a.mstat Writing sequence output to tmp_4-a-sort.a2m. @@@@ cat m0.a2m tmp_4-a-sort.a2m > tmp_4-a.train.seq @@@@ cat m0.cst tmp_4-a-md.cst > tmp_4-a.train.cst # About to count sequences in tmp_4-a.train.seq # num_seqs=122 nll_thresh=-18.5179889173466 frac_id=0.856179447944067 @@@@ uniqueseq thinned_tmp -alignfile tmp_4-a.train.seq -a protein \ -percent_id 0.856179447944067 -a2mdots 0 SAM: uniqueseq v3.2 (July 31, 2000) compiled 07/31/00_14:08:59 Reading alignment file tmp_4-a.train.seq (122 sequences, 184 columns) as A2M alignment. Writing sequence output to thinned_tmp.a2m. Dropping 8 (of 122) duplicate sequences with differing IDs Dropping 18 (of 122) sequences with > 85.6% id 96 sequences left after dropping 26 of 122 sequences. @@@@ modelfromalign junk_for_weights \ -alignfile tmp_4-a.train.seq \ -aweight_bits 0.5\ -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1 \ -aweight_method 1\ -aweight_exponent 10 SAM: modelfromalign v3.2 (July 31, 2000) compiled 07/31/00_14:08:27 Reading alignment file tmp_4-a.train.seq (96 sequences, 184 columns) as A2M alignment. @@@@ buildmodel tmp_4-b -insert tmp_4-a.mod\ -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1\ -modellength 0 \ -internal_weight 0 \ -anneal_length 2 \ -many_files 1 \ -Nseq 20000\ -nsurgery 0 \ -fracinsert 0.2 \ -cutinsert 0.25 -cutmatch 0.2 -initial_noise 0.0001 -anneal_noise 0.000001 \ -internal_weight 0 \ -train tmp_4-a.train.seq \ -nmodels 1\ -insert /projects/compbio/lib/fssp-trained.regularizer -sequence_weights tmp_4-a.w -constraints tmp_4-a.train.cst Reading parameter file tmp_4-a.mod tmp_4-a.mod(21): Reading MODEL -- Model from alignment file /projects/compbio/experiments/casp4/t115/t115-outer/T0115-outer.t2k_3.a2m Command line option(48): internal_weight has already been set. Using 0. Reading parameter file /projects/compbio/lib/fssp-trained.regularizer /projects/compbio/lib/fssp-trained.regularizer(1): Reading REGULARIZER: transitions from FSSP and pseudocounts for diverse weighted HSSP SAM: buildmodel v3.2 (July 31, 2000) compiled 07/31/00_14:08:12 -515.70 -28.14 -79.82 50.35 8 0 185 @@@@ hmmscore tmp_4-b -i tmp_4-b.mod -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1\ -dpstyle 0 \ -subtract_null 4 -sort 2 \ -db_size 551100 \ -db m0.a2m -db tmp_4-a.sel \ -select_score 4 \ -select_seq 4 \ -simple_threshold -22.5179889173466 -Emax 0.005 \ -select_mdalign 4 -select_md 4 \ -adpstyle 1 \ -Motifcutoff 0.108695652173913 \ -align_short 5 -mdEmax 0.005 -constraints tmp_4-a.train.cst -constraints_out /projects/compbio/experiments/casp4/t115/t115-outer/T0115-outer.t2k_4.cst Reading parameter file tmp_4-b.mod tmp_4-b.mod(18): Reading MODEL -- Final model for run tmp_4-b SAM: hmmscore v3.2 (July 31, 2000) compiled 08/04/00_11:31:33 Calculating Distances (. = 1000 sequences) . Average NLL-Simple NULL score: -87.129936 Database has 124 sequences with 39776 residues. @@@@ cp -f tmp_4-b.mult /projects/compbio/experiments/casp4/t115/t115-outer/T0115-outer.t2k_4.a2m @@@@ modelfromalign tmp_5-a -alignfile /projects/compbio/experiments/casp4/t115/t115-outer/T0115-outer.t2k_4.a2m \ -insert /projects/compbio/lib/fssp-trained.regularizer \ -aweight_bits 0.5\ -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1 \ -aweight_method 1\ -aweight_exponent 10 -constraints /projects/compbio/experiments/casp4/t115/t115-outer/T0115-outer.t2k_4.cst -constraints_out tmp_5-a.cst Reading parameter file /projects/compbio/lib/fssp-trained.regularizer /projects/compbio/lib/fssp-trained.regularizer(1): Reading REGULARIZER: transitions from FSSP and pseudocounts for diverse weighted HSSP SAM: modelfromalign v3.2 (July 31, 2000) compiled 07/31/00_14:08:27 Reading alignment file /projects/compbio/experiments/casp4/t115/t115-outer/T0115-outer.t2k_4.a2m (124 sequences, 184 columns) as A2M alignment. @@@@ hmmscore tmp_5-a -i tmp_5-a.mod -fimtrans -1\ -fimstrength 1\ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -binary_output 1\ -prior_library /projects/compbio/lib/recode3.20comp \ -a2mdots 0 \ -a protein \ -sw 2 -jump_in_prob 0.2 -jump_out_prob 1\ -dpstyle 0 \ -subtract_null 4 -sort 2 \ -db_size 551100 \ -simple_threshold -22.5179889173466 -Emax 0.005 \ -adpstyle 5 \ -align_short 0 -mdEmax 0.005\ -select_score 4 \ -select_seq 4 \ -select_mdalign 4 -select_md 4 -Motifcutoff 0.108695652173913 -db prefilter_4.fa -constraints tmp_5-a.cst -constraints_out tmp_5-a-md.cst Reading parameter file tmp_5-a.mod tmp_5-a.mod(21): Reading MODEL -- Model from alignment file /projects/compbio/experiments/casp4/t115/t115-outer/T0115-outer.t2k_4.a2m SAM: hmmscore v3.2 (July 31, 2000) compiled 08/04/00_11:31:33 Calculating Distances (. = 1000 sequences) . Average NLL-Simple NULL score: -27.855396 Database has 520 sequences with 142215 residues. @@@@ sortseq tmp_5-a-sort -alignfile tmp_5-a.mult \ -NLLfile tmp_5-a.mstat -a2mdots 0 SAM: sortseq v3.2 (July 31, 2000) compiled 07/31/00_14:08:48 Reading alignment file tmp_5-a.mult (121 sequences, 184 columns) as A2M alignment. Reading scores from file tmp_5-a.mstat Writing sequence output to tmp_5-a-sort.a2m. @@@@ cat m0.a2m tmp_5-a-sort.a2m > tmp_5-a.train.seq @@@@ cat m0.cst tmp_5-a-md.cst > tmp_5-a.train.cst @@@@ cp tmp_5-a.train.seq /projects/compbio/experiments/casp4/t115/t115-outer/T0115-outer.t2k.a2m @@@@ cp tmp_5-a.train.cst /projects/compbio/experiments/casp4/t115/t115-outer/T0115-outer.t2k.cst @@@@ rm -rf /projects/compbio/tmp/target2k-eclipse-21584 < /dev/null gzip -f T0115-outer.t2k.a2m T0115-outer.t2k_*.a2m prettyalign T0115-outer.t2k.a2m.gz -m8 -i -n -L3333 > T0115-outer.t2k.pa SAM: prettyalign v3.2 (July 31, 2000) compiled 08/11/00_16:27:51 gunzip -c T0115-outer.t2k.a2m.gz > tmp.a2m a2m2html -a2m_in tmp.a2m -retrieve > T0115-outer.t2k.pa.html SAM: /projects/compbio/bin/alpha/prettyalign v3.2 (July 31, 2000) compiled 08/11/00_16:27:51 rm tmp.a2m echo ReadNeuralNet /projects/compbio/usr/karplus/predict-2nd/testing/networks/overrep-2500-IDaa13-5-10-7-10-5-10-11-ehl2-seeded9-stride-trained.net > tmp.script echo ReadA2M T0115-outer.t2k.a2m.gz >> tmp.script echo PrintPrediction T0115-outer.t2k.2d 3670-4530-6947 >> tmp.script echo PrintPredictionFasta T0115-outer.t2k.2d.seq >> tmp.script echo PrintRDB T0115-outer.t2k.2d.rdb >> tmp.script predict-2nd < tmp.script # command:# Neural network set to overrep-2500-IDaa13-5-10-7-10-5-10-11-ehl2-seeded9-stride-trained.net # command:# Reading A2M format from T0115-outer.t2k.a2m.gz Bad base O for alphabet ExtAA # Using SequenceWeight EntropyWeight(1.4, 10) # Using regularizer /projects/compbio/lib/recode3.20comp for sequence weight regularizer. # T0115-outer.t2k.a2m with 122 sequences, total weight= 75.4612 avg weight= 0.618535 clipped 46 iterations # AdjustWeights couldn't save exactly 1.4 bits/position, saving 0.989171 bits. Regularizing alignment for T0115-outer.t2k.a2m.gz # After reading T0115-outer.t2k.a2m.gz, have 184 columns in 1 chains # command:# Initializing Gain for FinalLayer # Initializing Gain for ordering_layer # Initializing Gain for SecondLayer # Initializing Gain for FirstLayer # Network initialization done # Printing prediction to T0115-outer.t2k.2d # command:# Network initialization done # Printing prediction in FASTA format to T0115-outer.t2k.2d.seq # command:# Network initialization done # Printing prediction to T0115-outer.t2k.2d.rdb # command:rm tmp.script gunzip -f T0115-outer.t2k.a2m.gz /projects/compbio/usr/karplus/src/phytree/phytree -f -o -i -r flat T0115-outer.t2k T0115-outer.t2k.a2m /projects/compbio/lib/recode3.20comp No alphabet specified and file T0115-outer.t2k.a2m no help. Trying protein Prior library /projects/compbio/lib/recode3.20comp read. Reading alignment file T0115-outer.t2k.a2m (122 sequences) as A2M alignment. Alignment T0115-outer.t2k.a2m read. Adding sequences:0 10 20 30 40 50 60 70 80 90 100 110 120 One dot for every 10 merges: ............ Merges done. CPU = user 0: 0:57 system 0: 0: 0 gzip -f T0115-outer.t2k.a2m gzip -f T0115-outer.t2k_sorted.a2m rm T0115-outer.t2k.phytrace T0115-outer.t2k.tree_weight prettyalign T0115-outer.t2k_sorted.a2m.gz -m8 -i -n -L3333 > T0115-outer.t2k_sorted.pa SAM: prettyalign v3.2 (July 31, 2000) compiled 08/11/00_16:27:51 /projects/compbio/usr/karplus/src/phytree/dg T0115-outer.t2k.tree /projects/compbio/usr/karplus/src/phytree/dtree T0115-outer.t2k.tree make[1]: Leaving directory `/auto/projects/compbio/experiments/casp4/t115/t115-outer' echo "WeightingRegularizer /projects/compbio/lib/recode3.20comp" > tmp.script echo "SequenceWeight EntropyWeight 0.5 10.0" >> tmp.script echo "ReadA2M T0115-outer.t2k.a2m.gz" >> tmp.script echo "SavingsRegularizer /projects/compbio/lib/recode3.20comp" >> tmp.script echo "PrintSavings T0115-outer.t2k.saves" >> tmp.script echo "exit" >> tmp.script estimate-dist < tmp.script # command:Weighting regularizer set to recode3.20comp # command:Sequence weighting on input set to EntropyWeight(0.5, 10) # command:Reading A2M format from T0115-outer.t2k.a2m.gz Bad base O for alphabet ExtAA % using regularizer recode3.20comp % T0115-outer.t2k.a2m.gz with 122 sequences, total weight= 11.3378 avg weight= 0.0929331 After reading T0115-outer.t2k.a2m.gz, have 184 columns, totalling 1786.87 Using SequenceWeight EntropyWeight(0.5, 10) Using regularizer recode3.20compfor sequence weight regularizer. # command:Savings regularizer set to recode3.20comp # command:% Computing savings using regularizer recode3.20comp Printed 184 columns to T0115-outer.t2k.saves # command:rm tmp.script if test -e T0115-outer.t2k-logo.labels ;\ then makelogo -b 60 -t T0115-outer.t2k -o T0115-outer.t2k-logo.eps -l T0115-outer.t2k-logo.labels T0115-outer.t2k.saves ;\ else makelogo -b 60 -t T0115-outer.t2k -o T0115-outer.t2k-logo.eps -lf T0115-outer.t2k.2d.seq T0115-outer.t2k.saves ;\ fi stop = 131, bar_num = 121 119 131 H 0, 121 stopped stopped stopped stopped stopped stopped stopped stopped stopped stopped stopped stopped stopped stopped /projects/compbio/experiments/models.97/scripts99/score-target -alignment T0115-outer.t2k.a2m.gz -build w0.5 \ -db /projects/compbio/data/pdb/all-protein \ -Emax 40.0 \ -viterbi 0 -sw_score 2 \ -output T0115-outer.t2k-w0.5-pdb.rdb PATH=.:/projects/compbio/experiments/models.97/scripts99:/projects/compbio/bin:/projects/compbio/bin/alpha:/projects/compbio/bin/hmmscripts:/projects/compbio/bin/scripts:/sbin:/usr/sbin:/bin:/usr/bin:/usr/local/gnu/bin:/usr/local/share/request/bin:/usr/local/bin @@@@ mkdir /projects/compbio/tmp/score-target-6175 @@@@ mkdir /projects/compbio/tmp/score-target-6175/for-scorescript @@@@ /projects/compbio/experiments/models.97/scripts99/score-mods-vs-seqs.pl -root_tmp /projects/compbio/tmp/score-target-6175/for-scorescript -a2msfile /projects/compbio/tmp/score-target-6175/score-target.a2m \ -buildscript /projects/compbio/bin/scripts/w0.5 -seqfile /projects/compbio/data/pdb/all-protein -Emax 40.0 -viterbi 0 \ -sw_score 2 -output /projects/compbio/tmp/score-target-6175/score-target.op @@@@ chgrp protein /projects/compbio/tmp/score-target-6175/for-scorescript/split-tmp @@@@ chgrp protein /projects/compbio/tmp/score-target-6175/for-scorescript/split-tmp @@@@ /projects/compbio/bin/scripts/w0.5 /projects/compbio/experiments/casp4/t115/t115-outer/T0115-outer.t2k.a2m.gz /projects/compbio/tmp/score-target-6175/for-scorescript/split-tmp/eclipse-6127/T0115-outer.t2k.mod Reading /projects/compbio/bin/scripts/sam-t99.conf @@@@ chgrp protein /projects/compbio/tmp/tmp-build-weighted-model-eclipse-6234 @@@@ uniqueseq /projects/compbio/tmp/tmp-build-weighted-model-eclipse-6234/tmp -alignfile /projects/compbio/experiments/casp4/t115/t115-outer/T0115-outer.t2k.a2m.gz \ -a protein -percent_id 0.8 SAM: uniqueseq v3.2 (July 31, 2000) compiled 07/31/00_14:08:59 Reading alignment file /projects/compbio/experiments/casp4/t115/t115-outer/T0115-outer.t2k.a2m.gz (122 sequences, 184 columns) as A2M alignment. Writing sequence output to /projects/compbio/tmp/tmp-build-weighted-model-eclipse-6234/tmp.a2m. Dropping 7 (of 122) duplicate sequences with differing IDs Dropping 22 (of 122) sequences with > 80.0% id 93 sequences left after dropping 29 of 122 sequences. @@@@ modelfromalign /projects/compbio/tmp/tmp-build-weighted-model-eclipse-6234/tmp -alignfile /projects/compbio/tmp/tmp-build-weighted-model-eclipse-6234/tmp.a2m \ -insert /projects/compbio/lib/fssp-trained.regularizer \ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -prior_library /projects/compbio/lib/recode3.20comp \ -binary_output 1 -a protein -aweight_method 1 -aweight_bits 0.5 -aweight_exponent 10 Reading parameter file /projects/compbio/lib/fssp-trained.regularizer /projects/compbio/lib/fssp-trained.regularizer(1): Reading REGULARIZER: transitions from FSSP and pseudocounts for diverse weighted HSSP SAM: modelfromalign v3.2 (July 31, 2000) compiled 07/31/00_14:08:27 Reading alignment file /projects/compbio/tmp/tmp-build-weighted-model-eclipse-6234/tmp.a2m (93 sequences, 184 columns) as A2M alignment. @@@@ cp -f /projects/compbio/tmp/tmp-build-weighted-model-eclipse-6234/tmp.mod /projects/compbio/tmp/score-target-6175/for-scorescript/split-tmp/eclipse-6127/T0115-outer.t2k.mod @@@@ rm -rf /projects/compbio/tmp/tmp-build-weighted-model-eclipse-6234 80.0% id 93 sequences left after dropping 29 of 122 sequences. @@@@ modelfromalign /projects/compbio/tmp/tmp-build-weighted-model-eclipse-13717/tmp -alignfile /projects/compbio/tmp/tmp-build-weighted-model-eclipse-13717/tmp.a2m \ -insert /projects/compbio/lib/fssp-trained.regularizer \ -ins_jump_conf 1 \ -match_jump_conf 1 \ -del_jump_conf 1 \ -prior_library /projects/compbio/lib/recode3.20comp \ -binary_output 1 -a protein -aweight_method 1 -aweight_bits 0.5 -aweight_exponent 10 Reading parameter file /projects/compbio/lib/fssp-trained.regularizer /projects/compbio/lib/fssp-trained.regularizer(1): Reading REGULARIZER: transitions from FSSP and pseudocounts for diverse weighted HSSP SAM: modelfromalign v3.2 (July 31, 2000) compiled 07/31/00_14:08:27 Reading alignment file /projects/compbio/tmp/tmp-build-weighted-model-eclipse-13717/tmp.a2m (93 sequences, 184 columns) as A2M alignment. @@@@ cp -f /projects/compbio/tmp/tmp-build-weighted-model-eclipse-13717/tmp.mod T0115-outer.t2k-w0.5.mod @@@@ rm -rf /projects/compbio/tmp/tmp-build-weighted-model-eclipse-13717