CASP4 Target T0111

1. Protein Name: enolase
2. Organism Name: Escherichia coli
3. Number of amino acids (approx): 431
4. Accession number: P08324
5. Sequence Database: Swiss-prot
6. Amino acid sequence: SKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTGSREALEL
RDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTE
NKSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMM
NIINGGEHADNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKG
MNTAVGDEGGYAPNLGSNAEALAVIAEAVKAAGYELGKDITLAMDCAASE
FYKDGKYVLAGEGNKAFTSEEFTHFLEELTKQYPIVSIEDGLDESDWDGF
AYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKGIANSILIKFNQIGSLTE
TLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQIKTGSMSRSD
RVAKYNQLIRIEEALGEKAPYNGRKEIKGQA
7. Additional Information: oligomerization state: dimer in the presence of magnesium by dynamic light scattering and small angle x-ray solution scattering and
in the recently solved crystal structure.
8. Homologous Sequence of known structure: yes
9. Current state of the experimental work: Protein supply: overexpressed in E. coli
crystals: grown at 20 C from PEG 3550
diffraction quality: strong data to 2.5 A with good
redundancy

Structure solved by molecular replacement. Currently,
the refinement to 2.5 A resolution is near completion.
Current Rfree 27 % ; R 22 %
10. Interpretable map?: yes
11. Estimated date of chain tracing completion: complete
12. Estimated date of public release of structure: Dec 2000
13. Name: Unavailable until after public release of structure