CASP4 Target T0100

1. Protein Name

Pectin Methylesterase

2. Organism Name

Erwinia chrysanthemi

3. Number of amino acids (approx)

342

4. Accession number

P07863 PMEA_ERWCH

5. Sequence Database

Swiss-prot

6. Amino acid sequence

ATTYNAVVSK SSSDGKTFKT IADAIASAPA GSTPFVILIK NGVYNERLTI TRNNLHLKGE
SRNGAVIAAA TAAGTLKSDG SKWGTAGSST ITISAKDFSA QSLTIRNDFD FPANQAKSDS
DSSKIKDTQA VALYVTKSGD RAYFKDVSLV GYQDTLYVSG GRSFFSDCRI SGTVDFIFGD
GTALFNNCDL VSRYRADVKS GNVSGYLTAP STNINQKYGL VITNSRVIRE SDSVPAKSYG
LGRPWHPTTT FSDGRYADPN AIGQTVFLNT SMDNHIYGWD KMSGKDKNGN TIWFNPEDSR
FFEYKSYGAG AAVSKDRRQL TDAQAAEYTQ SKVLGDWTPT LP

7. Additional Information

Amino acids 1-24 are a leader sequence and are removed
Residue 80 is a His not a Leu
There are two gene sequences for strains 3937 and B374 which differ at two sites: residues 80 leu/His and 336 Ala/Thr. Unfortunately, the X-ray data clearly shows a compromise with His at 80 and Ala at 336! Crystals are at pH 6.5 in ammonium sulphate

8. Homologous Sequence of known structure

no

9. Current state of the experimental work

Structure completed and deposited on hold. Data to 2.4 A,
REMARK 3 R VALUE (WORKING SET) : 0.170
REMARK 3 FREE R VALUE : 0.212

10. Interpretable map?

yes

11. Estimated date of chain tracing completion

Done

12. Estimated date of public release of structure

July 5, 2000

13. Name

Unavailable until after public release of structure