CASP3 Target T0074
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1. Protein Name
- the second EH domain of eps15
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2. Organism Name
- human -- recombinant protein prepared in E.coli
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3. Number of amino acids (approx)
- 106
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4. Accession number
- P42566
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5. Sequence Database
- Swiss-prot
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6. Amino acid sequence
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DRWGS PWAVKPEDKA KYDAIFDSLS PVNGFLSGDK VKPVLLNSKL PVDILGRVWE LSDIDHDGML
DRDEFAVAMF LVYCALEKEP VPMSLPPALV PPSKRKTW LEI
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7. Homologous Sequence of known structure
- no
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8. Current state of the experimental work
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Structure has been determined by NMR and the paper has been accepted for publication,
it will appear in Science
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9. Interpretable map?
- yes
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10. Estimated date of chain tracing completion
- done
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11. Estimated date of public release of structure
- August 1998
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12. Additional Information
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The structure of EH2 was determined after subcloning amino acids 121-218
from human Eps15 in a prsetA vector, and was expressed in E.coli.
The protein contains 5 N-terminal residues (DRWGS) and three C-terminal
residues (ELI) from the expression vector.
About 30 EH domains in 20 proteins have been identified based on
amino acid sequence.
A calcium binding site is located in the current structure. The location
of this calcium binding site varies between EH domains.
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13. Name
- unavailable until after public release of structure
Note: Authors indicated that they are going to report both in paper
and in the PDB submission only the structure for residues 121-218 and skip
residues stemming from the expression vector (these residues were disordered in solution).
Therefore models for this target should contain only sequence 121-218 as
in template files below.
Related Files
Template Sequence file
Template PDB file