SWISS-PROT: P46849
ID   RTCA_ECOLI     STANDARD;      PRT;   339 AA.
AC   P46849; P46848; Q47349;
DT   01-NOV-1995 (REL. 32, CREATED)
DT   01-NOV-1997 (REL. 35, LAST SEQUENCE UPDATE)
DT   01-NOV-1997 (REL. 35, LAST ANNOTATION UPDATE)
DE   RNA 3'-TERMINAL PHOSPHATE CYCLASE (EC 6.5.1.4) (RNA-3'-PHOSPHATE
DE   CYCLASE) (RNA CYCLASE).
GN   RTCA.
OS   ESCHERICHIA COLI.
OC   PROKARYOTA; GRACILICUTES; SCOTOBACTERIA; FACULTATIVELY ANAEROBIC RODS;
OC   ENTEROBACTERIACEAE.
RN   [1]
RP   SEQUENCE FROM N.A.
RC   STRAIN=K12 / MG1655;
RA   BLATTNER F.R., PLUNKETT G. III, MAYHEW G.F., PERNA N.T., GLASNER F.D.;
RL   SUBMITTED (JAN-1997) TO EMBL/GENBANK/DDBJ DATA BANKS.
RN   [2]
RP   SEQUENCE OF 149-339 FROM N.A.
RC   STRAIN=K12;
RX   MEDLINE; 86275993. [NCBI, Geneva, Japan]
RA   COLE S.T., RAIBAUD O.;
RL   GENE 42:201-208(1986).
RN   [3]
RP   REVISION, AND CHARACTERIZATION.
RX   MEDLINE; 97327572. [NCBI, Geneva, Japan]
RA   GENSCHIK P., BILLY E., SWIANIEWICZ M., FILIPOWICZ W.;
RL   EMBO J. 16:2955-2967(1997).
CC   -!- FUNCTION: CATALYZES THE CONVERSION OF 3'-PHOSPHATE TO A 2',3'-
CC       CYCLIC PHOSPHODIESTER AT THE END OF RNA. THE MECHANISM OF ACTION
CC       OF THE ENZYME OCCURS IN 3 STEPS: (A) ADENYLATION OF THE ENZYME BY
CC       ATP; (B) THE ENZYME ACTS ON RNA-N3'P TO PRODUCE RNA-N3'PP5'A; (C)
CC       A NON CATALYTIC NUCLEOPHILIC ATTACK BY THE ADJACENT 2'HYDROXYL ON
CC       THE PHOSPHORUS IN THE DIESTER LINKAGE TO PRODUCE THE CYCLIC END
CC       PRODUCT. THE BIOLOGICAL ROLE OF THIS ENZYME IS UNKNOWN BUT IT IS
CC       LIKELY TO FUNCTION IN SOME ASPECTS OF CELLULAR RNA PROCESSING.
CC   -!- CATALYTIC ACTIVITY: ATP + RNA 3'-TERMINAL-PHOSPHATE = AMP +
CC       DIPHOSPHATE + RNA TERMINAL-2',3'-CYCLIC-PHOSPHATE.
CC   -!- SUBCELLULAR LOCATION: CYTOPLASMIC (POTENTIAL).
CC   -!- SIMILARITY: BELONGS TO THE RNA 3'-TERMINAL CYCLASE FAMILY.
CC   -!- CAUTION: REF.1 SEQUENCE DIFFERS FROM THAT SHOWN DUE TO A
CC       FRAMESHIFT IN POSITION 122 THAT PRODUCES TWO SEPARATE ORFS.
DR   EMBL; U18997; G606355; ALT_FRAME. [EMBL / GenBank / DDBJ] [CoDingSequence]
DR   EMBL; U18997; G606354; ALT_FRAME. [EMBL / GenBank / DDBJ] [CoDingSequence]
DR   EMBL; AE000418; G1789826; ALT_FRAME. [EMBL / GenBank / DDBJ] [CoDingSequence]
DR   EMBL; AE000418; G1789825; ALT_FRAME. [EMBL / GenBank / DDBJ] [CoDingSequence]
DR   EMBL; M13585; G1128963; -. [EMBL / GenBank / DDBJ] [CoDingSequence]
DR   ECOGENE; EG12938; RTCA.
DR   ECOCYC; EG12938; RTCA.
DR   PROSITE; PS01287; RTC; 1.
DR   PRODOM [Domain structure / List of seq. sharing at least 1 domain]
DR   PROTOMAP; P46849.
DR   SWISS-2DPAGE; GET REGION ON 2D PAGE.
KW   LIGASE.
SQ   SEQUENCE   339 AA;  36034 MW;  DCE616E7 CRC32;
     MMKRMIALDG AQGEGGGQIL RSALSLSMIT GQPFTITSIR AGRAKPGLLR QHLTAVKAAT
     EICGATVEGA ELGSQRLLFR PGTVRGGDYR FAIGSAGSCT LVLQTVLPAL WFADGPSRVE
     VSGGTDNPSA PPADFIRRVL EPLLAKIGIH QQTTLLRHGF YPAGGGVVAT EVSPVASFNT
     LQLGERGNIV QMRGEVLLAG VPRHVAEREI ATLAGSFSLH EQNIHNLPRD QGPGNTVSLE
     VESENITERF FVVGEKRVSA EVVAAQLVKE VKRYLASTAA VGEYLADQLV LPMALAGAGE
     FTVAHPSCHL LTNIAVVERF LPVRFSLIET DGVTRVSIE
//

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