LOCUS       2507354       339 aa                              01-NOV-1997
DEFINITION  RNA 3'-TERMINAL PHOSPHATE CYCLASE (RNA-3'-PHOSPHATE CYCLASE) (RNA
            CYCLASE).
ACCESSION   2507354
PID         g2507354
DBSOURCE    SWISS-PROT: locus RTCA_ECOLI, accession P46849
            class: standard.
            extra accessions:P46848,Q47349,created: Nov 1, 1995.
            sequence updated: Nov 1, 1997.
            annotation updated: Nov 1, 1997.
            xrefs: gi: 606010, gi: 606355, gi: 2367222, gi: 1789826, gi:
            146716, gi: 1128963
            xrefs (non-sequence databases): ECOGENE EG12938, PROSITE PS01287
KEYWORDS    LIGASE.
SOURCE      Escherichia coli.
  ORGANISM  Escherichia coli
            Eubacteria; Proteobacteria; gamma subdivision; Enterobacteriaceae;
            Escherichia.
REFERENCE   1  (residues 1 to 339)
  AUTHORS   BLATTNER,F.R., PLUNKETT,G. III, MAYHEW,G.F., PERNA,N.T. and
            GLASNER,F.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (??-JAN-1997) TO EMBL/GENBANK/DDBJ DATA BANKS
  REMARK    SEQUENCE FROM N.A.
            STRAIN=K12 / MG1655
REFERENCE   2  (residues 1 to 339)
  AUTHORS   Cole,S.T. and Raibaud,O.
  TITLE     The nucleotide sequence of the malT gene encoding the positive
            regulator of the Escherichia coli maltose regulon
  JOURNAL   Gene 42 (2), 201-208 (1986)
  MEDLINE   86275993
  REMARK    SEQUENCE OF 149-339 FROM N.A.
            STRAIN=K12
REFERENCE   3  (residues 1 to 339)
  AUTHORS   Genschik,P., Billy,E., Swianiewicz,M. and Filipowicz,W.
  TITLE     The human RNA 3'-terminal phosphate cyclase is a member of a new
            family of proteins conserved in Eucarya, Bacteria and Archaea
  JOURNAL   EMBO J. 16 (10), 2955-2967 (1997)
  MEDLINE   97327572
  REMARK    REVISION, AND CHARACTERIZATION.
COMMENT     [FUNCTION] CATALYZES THE CONVERSION OF 3'-PHOSPHATE TO A
            2',3'-CYCLIC PHOSPHODIESTER AT THE END OF RNA. THE MECHANISM OF
            ACTION OF THE ENZYME OCCURS IN 3 STEPS: (A) ADENYLATION OF THE
            ENZYME BY ATP; (B) THE ENZYME ACTS ON RNA-N3'P TO PRODUCE
            RNA-N3'PP5'A; (C) A NON CATALYTIC NUCLEOPHILIC ATTACK BY THE
            ADJACENT 2'HYDROXYL ON THE PHOSPHORUS IN THE DIESTER LINKAGE TO
            PRODUCE THE CYCLIC END PRODUCT. THE BIOLOGICAL ROLE OF THIS ENZYME
            IS UNKNOWN BUT IT IS LIKELY TO FUNCTION IN SOME ASPECTS OF CELLULAR
            RNA PROCESSING.
            [CATALYTIC ACTIVITY] ATP + RNA 3'-TERMINAL-PHOSPHATE = AMP +
            DIPHOSPHATE + RNA TERMINAL-2',3'-CYCLIC-PHOSPHATE.
            [SUBCELLULAR LOCATION] CYTOPLASMIC (POTENTIAL).
            [SIMILARITY] BELONGS TO THE RNA 3'-TERMINAL CYCLASE FAMILY.
            [CAUTION] REF.1 SEQUENCE DIFFERS FROM THAT SHOWN DUE TO A
            FRAMESHIFT IN POSITION 122 THAT PRODUCES TWO SEPARATE ORFS.
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Escherichia coli"
                     /db_xref="taxon:562"
                     1..339
     Protein         1..339
                     /product="RNA 3'-TERMINAL PHOSPHATE CYCLASE"
                     /EC_number="6.5.1.4"
ORIGIN      
        1 mmkrmialdg aqgegggqil rsalslsmit gqpftitsir agrakpgllr qhltavkaat
       61 eicgatvega elgsqrllfr pgtvrggdyr faigsagsct lvlqtvlpal wfadgpsrve
      121 vsggtdnpsa ppadfirrvl epllakigih qqttllrhgf ypagggvvat evspvasfnt
      181 lqlgergniv qmrgevllag vprhvaerei atlagsfslh eqnihnlprd qgpgntvsle
      241 veseniterf fvvgekrvsa evvaaqlvke vkrylastaa vgeyladqlv lpmalagage
      301 ftvahpschl ltniavverf lpvrfsliet dgvtrvsie
//

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