Abstract: We have determined the complete 1,694,969-nt sequence of the GC-rich genome of Methanopyrus kandleri by using a whole direct genome sequencing approach. This approach is based on unlinking of genomic DNA with the ThermoFidelase version of M. kandleri topoisomerase V and cycle sequencing directed by 2'-modified oligonucleotides (Fimers). Sequencing redundancy (3.3x) was sufficient to assemble the genome with less than one error per 40 kb. Using a combination of sequence database searches and coding potential prediction, 1,692 protein-coding genes and 39 genes for structural RNAs were identified. M. kandleri proteins show an unusually high content of negatively charged amino acids, which might be an adaptation to the high intracellular salinity. Previous phylogenetic analysis of 16S RNA sugg... [Click above reference link for full abstract]
A genome position can be specified by chromosomal coordinate range, COG
ID, or keywords from the GenBank or TIGR description of a gene.
The available chromosome/plasmid names are:
Browser Chrom/Plasmid Name | Length (bp) | GC Content (%) | Gene Count | NCBI RefSeq Accession |
---|---|---|---|---|
chr | 1694969 | 61.16 | 1729 | NC_003551 |
The following list shows examples of valid position queries for this genome:
Request: | Genome Browser Response: |
---|---|
chr | Displays the entire sequence "chr" in the browser window |
chr:1-10000 | Displays first ten thousand bases of the sequence "chr" |
transporter | Lists all genes with "transporter" in the name or description |
MK0010 | Display genome at position of gene MK0010 |
If you use the browser in your published research, please cite our publication in the Nucleic Acids Research Database Issue. Citations and positive feedback will help us obtain funding to continue development of this community resource.