Abstract: Many important virulence genes of pathogenic bacteria are preferentially expressed in vivo. We used the recently developed in vivo-induced antigen technology (IVIAT) to identify Vibrio vulnificus genes induced in vivo. An expression library of V. vulnificus was screened by colony blot analysis by using pooled convalescent-phase serum that had been thoroughly adsorbed with in vitro-expressed V. vulnificus whole cells and lysates. Twelve clones were selected, and the sequences of the insert DNAs were analyzed. The DNA sequences showed homologies with genes encoding proteins of diverse functions: these functions included chemotaxis (a methyl-accepting chemotaxis protein), signaling (a GGDEF-containing protein and a putative serine/threonine kinase), biosynthesis and metabolism (PyrH, PurH, an... [Click above reference link for full abstract]
A genome position can be specified by chromosomal coordinate range, COG
ID, or keywords from the GenBank or TIGR description of a gene.
The available chromosome/plasmid names are:
Browser Chrom/Plasmid Name | Length (bp) | GC Content (%) | Gene Count | NCBI RefSeq Accession |
---|---|---|---|---|
chrI | 3281945 | 46.45 | 3049 | NC_004459 |
chrII | 1844853 | 47.12 | 1575 | NC_004460 |
The following list shows examples of valid position queries for this genome:
Request: | Genome Browser Response: |
---|---|
chrI | Displays the entire sequence "chrI" in the browser window |
chrI:1-10000 | Displays first ten thousand bases of the sequence "chrI" |
transporter | Lists all genes with "transporter" in the name or description |
VV10010 | Display genome at position of gene VV10010 |
If you use the browser in your published research, please cite our publication in the Nucleic Acids Research Database Issue. Citations and positive feedback will help us obtain funding to continue development of this community resource.