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A Computational Screen for Methylation Guide snoRNAs in Yeast
4.1
Abstract
Introduction
Experimental Procedures
snoRNA Search Algorithm and Model Scoring
snoRNA Gene Disruptions
Mapping of rRNA Ribose Methylations by Primer Extension
Verification of snoRNA Transcription and 5'-ends
Results
Computer Search Algorithm and Probabilistic snoRNA Model
snoRNAs Assigned to 39 of 42 Known Ribose Methyl Sites
snoRNAs Assigned to 12 of 13 Previously Unmapped Ribose Methyl Sites
Expression and 5' ends of New Yeast snoRNAs Verified
snR70 Identified by Comparative Genomics
Discussion
snoRNAs Assigned to 51 of 55 Total Ribose Methyl Sites
A Nearly Complete Set of Methylation Guide snoRNAs in Yeast
Multiple Methylations Guided by Single snoRNAs
Methylation Guide snoRNA Consensus Structure
Genomic Organization of the snoRNA Gene Family
Implications for Genome Sequence Analysis
Acknowledgments
Data availability
Todd M. Lowe
2000-03-31
