CreatePredAlphaCost pred_alpha2k alpha11 T0220.t2k.alpha.rdb 2 alpha alpha_prev sum
CreatePredAlphaCost pred_alpha04 alpha11 T0220.t04.alpha.rdb 2 alpha alpha_prev sum

SetCost wet6.5 3 near_backbone 3 way_back 3 dry5 5 dry6.5 6 dry8 5 dry12 2 \
	phobic_fit 2 \
	sidechain 6 \
	bystroff 5 \
	soft_clashes 10  backbone_clashes 2 \
	break 20  \
	pred_alpha2k 3 \
	pred_alpha04 3 \
	constraints 10 \
	hbond_geom 0.1 \
	hbond_geom_backbone 0.1 \
	hbond_geom_beta 0.4 \
	hbond_geom_beta_pair 1

// Add the following for extra-cellular proteins:
#	known_ssbond 0.5	# and put ssbond command in constraints file
#	maybe_ssbond 0.5

// For comparative modeling, increase weight of break and hbond_geom...

// put any constraints here, such as helix constraints, strand constraints,
//	sheet constraints, ssbonds, hbonds, or just arbitrary pairs of atoms.

include T0220.t2k.dssp-ehl2.constraints
include T0220.t2k.dssp-ebghstl.constraints
include T0220.t2k.stride-ebghtl.constraints
include T0220.t2k.str2.constraints
include T0220.t2k.alpha.constraints
include T0220.t2k.bys.constraints

include T0220.t04.dssp-ehl2.constraints
include T0220.t04.dssp-ebghstl.constraints
include T0220.t04.stride-ebghtl.constraints
include T0220.t04.str2.constraints
include T0220.t04.alpha.constraints
include T0220.t04.bys.constraints

# The following sheet constraints are not entirely consistent.
# Downweight the inconsistent ones and thin on the next iteration

#from 1gpjA
SheetConstraint D189 T194	I213 A218	hbond L191	0.1
SheetConstraint F128 G132	V190 T194	hbond G129
SheetConstraint V127 G132	E152 D157	hbond F128
SheetConstraint V153 A155	I175 A177	hbond A155
SheetConstraint V201 K203	K223 E225	hbond V202
SheetConstraint H212 I216	K233 V237	hbond I213	0.1

#from 1nytA
# SheetConstraint T80 M82	Y103 T105	hbond V81
# SheetConstraint G93 F94	R108 L107	hbond F94
# SheetConstraint F94 V98	S106 T102	hbond L96
SheetConstraint D189 T193	T211 A215	hbond L191	0.3
SheetConstraint V127 G132	D189 T194	hbond G129
SheetConstraint F128 G132	V153 D157	hbond F130
SheetConstraint I150 E152	S126 F128	hbond G151
SheetConstraint V153 A155	I175 A177	hbond V153

#from try1-opt2
# SheetConstraint A62 K65	D100 A97	hbond L64
# SheetConstraint L96 R108	R108 L96	hbond A97
SheetConstraint D189 T193	T211 A215	hbond L191	0.3
SheetConstraint F128 G132	V190 T194	hbond G129
SheetConstraint F128 G132	V153 D157	hbond F128
SheetConstraint V153 A155	I175 A177	hbond A155
SheetConstraint H212 A215	I236 D239	hbond I213	0.3